核农学报2018，Vol.32Issue(6)：1070-1078,9.DOI:10.11869/j.issn.100-8551.2018.06.1070
自然高低温胁迫下粳稻穗粒数相关性状稳定位点的发掘
Mining Stable Locus at Natural High Temperature and Low Temperature Stress of Grain Number Related Traits in Japonica Rice
摘要
Abstract
To explore stable locus under natural high temperature and low temperature stress of grain number three related-traits in japonica.Unconditional QTL analysis and conditional QTL analysis by adjusted to an identical level of growth duration were conducted for grain number per panicle (GNP),filled grain number per panicle (FGP) and percentage of spikelet fertility (SF) in a set of chromosome segment substitution lines (CSSLs) derived from a japonica cross of Nipponbare (recipient) and Koshihikari (donor).The 48-line CSSL population was planted under normal temperature year of 2015 in Lujiang(E1),low temperature year of 2016 in Lingshui(E2) and high temperature year of 2016 in Lujiang (E3).Results showed that all QTLs for GNP,FGP and SF detected by the two methods were predominant additive effects.Stable loci of qGNP7.1 of GNP,qFGP6.1 and qFGP11 of FGP and qSF9 and qSF11 of SF were detected.Allele from Nipponbare can increase effect in qFGP6.1 and qSF9.Allele from Koshihikari can increase effect in qGNP7.1,qFGP11 and qSF11,qFGP1 1 and qSF11 on the same chromosome segment were detected in three environments.The result indicated that alleles of these loci could be stably expressed under natural high temperature and low temperature,qGNP7.1 was detected in E1 and E2 showed that expression of allele from the locus was unaffected under low natural temperature,qFGP6.1 and qSF9 were detected in E1 and E3 showed that expression of alleles from these two loci were unaffected at natural high temperature.These results will provide valuable reference information for improving grain number related-traits by molecular markers and breeding variety with natural high and low temperature stress.关键词
粳稻/自然高低温胁迫/穗粒数/条件QTL定位/稳定位点Key words
japonica rice/natural high temperature and low temperature stress/grain number/conditional QTL mapping/stable locus引用本文复制引用
江建华,吴爽,胡长敏,景春雨,王德正..自然高低温胁迫下粳稻穗粒数相关性状稳定位点的发掘[J].核农学报,2018,32(6):1070-1078,9.基金项目
国家自然科学基金项目(31601374),安徽省科技计划项目(1501031096),安徽省水稻遗传育种重点实验室绩效项目(2016SDSY004) （31601374）
