核农学报2018，Vol.32Issue(6)：1089-1096,8.DOI:10.11869/j.issn.100-8551.2018.06.1089
换锦花EST-SSR标记开发及遗传多样性分析
Development of EST-SSR Markers and Genetic Diversity Analysis in Lycoris sprengeri
摘要
Abstract
In order to develop EST-SSR markers and analysis genetic diversity of Lycoris sprengeri.MISA was used to detect the SSR locus of Lycoris sprengeri transcriptome,and primers were designed by Primer3.0.The effectiveness and polymorphism of primers were determined by PCR amplification and polyacrylamide gel electrophoresis,then draw the dendrogram of Lycoris sprengeri.The results show that,9 918 SSR loci were detected,the occurrence frequency is 18.59％ and the distribution distance is 3.729 Kb.The repeat units of SSR loci are 1 ～ 3 bases,in which single base repeats ＞ three base repeats ＞ two base repeats,and accounted for 61.08％,23.22％ and 14.23％ respectively,while 4 base and more repeat units are relatively less.A total of 9 302 pairs of SSR primers were designed and 278 pairs of primers were randomly selected and amplified by PCR.There were 185 pairs of primers that were clearly amplified (66.55％).Of the seven species of Lycoris,59 were polymorphic (31.89％).Among them,11 pairs (18.64％)showed rich polymorphism in 30 accessions of Lycoris sprengeri in Zhejiang and Jiangsu.The EST-SSR markers developed in this study are rich in polymorphism,it is of great significance in the genetic diversity analysis,identification of hybrid species and the construction of genetic map for the Lycoris sprengeri and the Lycoris plants.关键词
换锦花/转录组/EST-SSR/多态性/遗传多样性Key words
Lycoris sprengeri/transcriptome/EST-SSR/polymorphism/genetic diversity引用本文复制引用
石艳,童再康,高燕会..换锦花EST-SSR标记开发及遗传多样性分析[J].核农学报,2018,32(6):1089-1096,8.基金项目
国家自然科学基金项目(31670696),浙江省“十三五”林木新品种选育专项(2016C02056) （31670696）
