核农学报2018，Vol.32Issue(6)：1153-1161,9.DOI:10.11869/j.issn.100-8551.2018.06.1153
铜藻多糖酶解工艺优化及肠道环境调节功能研究
Enzyme Process Optimization of Sargassum horueri Polysaccharide and Regulation Function of Intestinal Environment Research
摘要
Abstract
In order to investigate the optimum enzymatic hydrolysis conditions of Sargassum horueri polysaccharide (SHP) under the action of polysaccharide enzyme and the regulation function of intestinal environment of polysaccharide components with different average molecular weight,response surface methodology was used to optimize the enzymatic hydrolysis process of SHP,as well as to investigate the effect of polysaccharide on the intestinal environment of mice.The results showed that the optimal enzymatic hydrolysis parameters were determined as follows:enzymolysis temperature 48℃,enzyme concentration 3.6％,pH value 4.8,enzymolysis time 2 h.Under these conditions,the content ofreducing sugars was 0.550 mg· mL-1,which was consistent with the theoretical value.SHPE1,SHPE2,SHPE3 were obtained by the purification of Sargassum horueri polysaccharide enzymatic hydrolysates (SHPE),and SHPO was obtained from SHP purified by column chromatography system.The intestinal microflora group regulating function test showed that the Sargassum horueri polysaccharide components exhibited a certain intestinal probiotic effect.Among them,the effects of SHPE2 and SHPE3 with relative low molecular weight on the intestinal pH value,the total volatile basic nitrogen (TVB-N) content and other intestinal probiotics indicators in mice were close to fructooligosaccharides (FOS).This study informed that Sargassum horueri polysaccharide components had significant potential to be developed into a marine algae prebiotics.关键词
铜藻/多糖/酶解/益生元Key words
Sargassum horueri/polysaccharides/enzymolysis/prebiotics引用本文复制引用
严志鹏,张皓雅,涂红艳,陈晓晓,邵平..铜藻多糖酶解工艺优化及肠道环境调节功能研究[J].核农学报,2018,32(6):1153-1161,9.基金项目
国家自然科学基金(31301560) （31301560）
