核农学报2018，Vol.32Issue(6)：1211-1219,9.DOI:10.11869/j.issn.100-8551.2018.06.1211
镉、汞胁迫对桔梗种子萌发、幼苗生理生化特性及镉、汞含量的影响
Respones of Seed Germination and Physiological, Biochemical Characteristics and Cd, Hg Content of Platycodon grandiflorum Seedling to Cadmium and Mercury Stress
摘要
Abstract
To investigate effect of heavy mental on seed germination,seedling growth and physiological,biochemical characteristics of plant,the response of seed germination,seedling growth and physiological,biochemical characteristics of Platycodon grandiflorum under different concentrations of cadmium and mercury stress were studied by mean of petri dish filter paper method.The results showed that low concentration of Cd2+ or Hg2+ (≤20 mg· L-1) treatment had little effect on the germination energy and germination rate,while the germination energy and germination rate dropped significantly when the concentration of Cd2+ was higher than 30 mg· L-1 and the concentration of Hg2+ was higher than 40 mg· L-1.The germination index dropped when the concentration of Cd2+ was higher than 20mg· L-1 and the concentration of Hg2+ was higher than 30 mg· L-1,and the vitality index dropped with the increasing of the Cd2+ and Hg2 + concentration.The root length,plant height and fresh weight was reduced with the increasing of the Cd2+ and Hg2+ concentration.The soluble sugar content increased first decreased then with the increasing of the Cd2+ and Hg2+ concentration,however the soluble protein content increased first,then and increased finally with the increasing of the Cd2+ concentration,but increased with the Hg2 + concentration and were keeping at high level.The MDA content dropped first increased then after by Cd2+ treatment and incrased after by Hg2+ trteatment.The SOD activity rose first then declined with the increasing of the Cd2+ and Hg2+ concentration,while the POD activity rose first,then declined and rose at last with the increasing of the Cd2+ concentration,declined first then rose with the increasing of the Hg2+ concentration.The Cd,Hg content of the above ground and root increased with the increasing of the Cd2+ and Hg2+ concentration.In conclusion,the low concentration of Cd2 + and Hg2 + has little effects on seed germination but inhibited the growth of seedlings,higher concentration of Cd2+ and Hg2+ inhibited the seed germination and seedling growth,biochemical changes and increased the Cd,Hg content.This study provides a theoretical basis to elucidate the mechanism of heavy metal stress to seedling growth of Platycodon grandiflorum.关键词
桔梗/镉/汞/种子萌发/生理生化特性Key words
Platycodon/grandiflorum/cadmium/mercury/seed/germination/physiological/and/biochemical characteristics引用本文复制引用
尚宏芹,高昌勇..镉、汞胁迫对桔梗种子萌发、幼苗生理生化特性及镉、汞含量的影响[J].核农学报,2018,32(6):1211-1219,9.基金项目
山东省自然科学基金(ZR2015CL008),菏泽学院植物生物学重点实验室资助项目 （ZR2015CL008）
