湖南中医药大学学报2018，Vol.38Issue(4)：372-375,4.DOI:10.3969/j.issn.1674-070X.2018.04.002
新型造模法制备功能性消化不良肝郁脾虚证大鼠模型
A New Method for Building the Model of Functional Dyspepsia Rats with Liver Depression and Spleen Deficiency Syndrome
摘要
Abstract
Objective From the delay of gastric emptying, visceral hypersensitivity, psychological factors and other causes to improve the establishment of a new animal model of dyspepsia rats with liver depression and spleen deficiency syndrome. Methods The SD rats were randomly divided into five groups: high fat group (group A), rhubarb group (group B), combination group (group C), clip tail control group (group D), blank control group (group E). Except for the blank control group (group E), the remaining four groups were given the basis training of restraint and swimming. In group A, the high fat caused delay of gastric emptying was added. In group B, bitter cold diarrhea decoction caused spleen deficiency was added, and group C was formed by combination of group A and group B. Group D as a control group was given a commonly used modeling method of tailgating stimulation. The modeling results were evaluated by observing behavioral status, gastric emptying rate, small intestine propulsion rate. Results After modeling, rats in group A, B, C and D appeared the behavior of getting together, keeping to the side, decreased activity, reduced aggression, vulnerable to scared and irritable, hair dull and dull, and soft stool. Compared with group E, the gastric empting rate and small intestine advance rate in other four groups decreased, and the decrease degree in group C and D was more obvious, the differences were statistically significant (P<0.01). Conclusion The compounding method could make rats appear a series of symptoms of liver depression and spleen deficiency, and effectively reduce the gastric emptying rate and intestinal propulsion rate in rats.关键词
功能性消化不良/肝郁脾虚证/动物模型Key words
functional dyspepsia/liver depression and spleen deficiency/animal models分类
医药卫生引用本文复制引用
朱洁,王叶,郭璇,刘乐平,韩志飞..新型造模法制备功能性消化不良肝郁脾虚证大鼠模型[J].湖南中医药大学学报,2018,38(4):372-375,4.基金项目
国家自然科学基金项目(81403384) （81403384）
湖南省教育厅优秀青年项目(15B181) （15B181）
湖南中医药大学校级创新性实验课题(30). （30）
