湖南中医药大学学报2018，Vol.38Issue(4)：376-378,3.DOI:10.3969/j.issn.1674-070X.2018.04.003
小鼠阴道涂片技术及其染色方法的研究
Study of Vaginal Smear and Staining Methods of Mice
摘要
Abstract
Objective To compare the staining effect of HE staining, Wright staining and Pap staining on the cell smear of C57BL/6 mice, and to find a relatively simple and effective staining method for identification of estrous cycle. Methods The 10 female C57BL/6 mice were taken vaginal smear at 10:00 every morning, with the course of 15 days. The results were analyzed by HE staining, Wright staining and Papanicolaou stainining methods. Results The normal saline method was better than swab method. HE staining method was simple and easy. Wright staining method was clear with obvious color separation. Papanicolaou stainining was with good diaphaneity. Conclusion The Wright staining method is firstly selected in large sample examination of mice vaginal smear for estimating the development of estrous cycle. The reliability of experimental information can be supplemented by Papanicolaou stainining method.关键词
C57BL/6/动情周期/阴道涂片/HE染色/瑞氏染色/巴氏染色Key words
C57BL/6/estrous cycle/vaginal smear/HE staining/Wright staining/Papanicolaou stainining分类
医药卫生引用本文复制引用
肖小芹,汤婉婷,成绍武,邓桂明..小鼠阴道涂片技术及其染色方法的研究[J].湖南中医药大学学报,2018,38(4):376-378,3.基金项目
湖南省中医科研计划重点项目(201815) （201815）
湖南省教育厅项目(17C1219). （17C1219）
