湖南中医药大学学报2018，Vol.38Issue(4)：379-383,5.DOI:10.3969/j.issn.1674-070X.2018.04.004
HMGB1介导NF-kB通路促进多囊卵巢综合征胰岛素抵抗细胞模型的凋亡
HMGB1 Mediates NF-kB Pathway in Promoting the Apoptosis of Insulin Resistant Cell Model of Polycystic Ovary Syndrome
摘要
Abstract
Objective To investigate the effect of HMGB1 on IkB/NF-KB signaling pathway in ovarian granulosa cells of rats with insulin resistant. Methods The ovarian granulosa cells in vitro were cultured, and the control group, different concentrations of insulin intervention groups, different concentrations of HMGB1 intervention groups and insulin resistance+si-HMGB1 group and others were designed. The levels of glucose, HMGB1, androgen, TNF-α and phosphorylated NF-kB p65 were determined by enzyme-linked immuno sorbent assay (ELISA). The concentrations of IkBa, pIkBa, Caspase3 and Cleaved Casepase3 were detected by Western blotting. Results Compared with the control group, high insulin significantly decreased the glucose of rats (P<0.05), increased the androgen, HMGB1 and TNF-α (P<0.01) in ovarian granulosa cells, and then activated transcription factor NF-kB p65, significantly increased phosphorylated NF-kB p65(P<0.05). Androgen, phosphorylated IKBa, phosphorylated NF-kB p65 and TNF-a increased significantly with the increase of HMGB1 (P<0.05 or P<0.01). When adding HMGB1 interference small molecules into the ovarian granulosa cells, side effects induced by insulin resistance relieved, and the expression of androgen, Cleaved caspase 3, TNF-α, phosphorylated IkBa and phosphorylated NNF-kB p65(P<0.05 or P<0.01) were significantly lower than the control group. Conclusion HMGB1 could promote the increase of inflammatory factors in the PCOS insulin resistance ovarian granulosa cell model. HMGB1 may mediate the NF-kB signaling pathway, promote the cell apoptosis in insulin resistance ovarian granulosa cells, also involve in the pathogenesis of PCOS.关键词
高迁移率族蛋白1/多囊卵巢综合征/胰岛素抵抗/卵巢颗粒细胞Key words
polycystic ovary syndrome/high mobility group protein 1/insulin resistance/ovarian granulosa cells分类
医药卫生引用本文复制引用
王隆卉,管宇,倪晓容..HMGB1介导NF-kB通路促进多囊卵巢综合征胰岛素抵抗细胞模型的凋亡[J].湖南中医药大学学报,2018,38(4):379-383,5.基金项目
上海市自然科学基金项目(17ZR1427900) （17ZR1427900）
上海市卫生局和计划生育委员会科研课题(201540087). （201540087）
