湖南中医药大学学报2018，Vol.38Issue(4)：389-392,4.DOI:10.3969/j.issn.1674-070X.2018.04.006
补肾药膳方对绝经后骨质疏松症模型大鼠CTX-Ⅰ指标及骨组织形态的影响
Effect of Reinforcing Kidney Medicinal Diet on CTX-ⅠIndex and Bone Morphology in Postmenopausal Osteoporosis Model Rats
摘要
Abstract
Objective To observe the effect of reinforcing kidney medicinal diet on CTX-Ⅰindex and bone morphological changes in postmenopausal osteoporosis (PMOP) model rats. Methods SPF female SD rats were randomly divided into normal group, sham operation group, model blank group, high dose group, low dose group and Gushukang group, with 10 rats in each group. Except normal group and sham operation group, other rats were ovariectomized. After 15 days of adaptive feeding, the rats were given intervention treatment for 12 weeks. The expression of CTX-Ⅰ in serum was detected by ELISA method. Bone morphology was observed by HE staining. Results Compared with the normal group and sham-operation group,the concentration of CTX-Ⅰin model blank group, high dose group, low dose group, Gushukang group increased significantly (P<0.05). Compared with the model blank group, the concentration of CTX-Ⅰ in high dose group, low dose group and Gushukang group decreased significantly (P<0.05). CTX-1 in low dose group was lower than the high dose group and Gushukang group, but the difference between them was not significant (P>0.05). In the bone morphology, the trabecular bone of the model blank group was significantly thinner, with obvious fracture, twisting, poor shape and structure, increased gap between the trabecular bone and bone marrow cavity. The bone marrow cavity expanded and merged obviously. The high dose group, the low dose group and the Gushukang group could improve bone morphology to some extent. Conclusion The effect of reinforcing kidney medical diet on the concentration of CTX-I is comparable with that of Gushukang granules. It could reduce the level of bone resorption markers, reduce bone resorption, improve bone shape and alleviate osteoporosis to a certain extent. The accuracy of dose should be further studied.关键词
补肾药膳方/绝经后骨质疏松/CTX-Ⅰ指标/骨形态Key words
rreinforcing kidney medicinal diet/postmenopausal osteoporosis/CTX-Ⅰindex/bone morphology分类
医药卫生引用本文复制引用
林静,张洁,陈燕..补肾药膳方对绝经后骨质疏松症模型大鼠CTX-Ⅰ指标及骨组织形态的影响[J].湖南中医药大学学报,2018,38(4):389-392,4.基金项目
湖南省科技厅课题(2015SK20168) （2015SK20168）
湖南中医药大学2017年一方研究生科研创新项目(YF201712). （YF201712）
