湖南中医药大学学报2018，Vol.38Issue(4)：397-401,5.DOI:10.3969/j.issn.1674-070X.2018.04.008
不同浓度的石榴皮多酚软膏对兔耳粉刺模型组织病理形态学的影响
Study on the Preparation of Pomegranate Peel Polyphenol Ointment and its Effect on the Pathomorphology of Rabbit Ear Acne Model
摘要
Abstract
Objective To observe the different concentration of pomegranate peel polyphenols ointment on histopathologic morphology in rabbit ear acne model, and to determine the best concentration of pomegranate peel polyphenols ointment on acne. Methods The 2% coal tar daub solution about 0.5 mL was smeared at about 2.0 cm x 2.0 cm range of rabbit left and right outer edge, once daily for two weeks to make rabbit ear acne model. The different concentration (18%, 9%, 3%) of pomegranate peel polyphenols ointment was prepared, which was smeared evenly on the rabbit ear acne model, twice daily for four weeks. The histopathologic changes were observed under light microscopy. Results The medium dose of pomegranate peel polyphenols group and the control group (isotretinoin) showed the most obvious effect on the pathological histology of acne model in the degree of inflammation, keratinization, hair follicles expansion. Compared with blank group, the degree of inflammation, keratinization, hair follicles expansion of model group was more significant (P <0.01). Compared with the model group, the medium dose of pomegranate peel polyphenols group and the control group could significantly decrease the degree of inflammation, keratinization, hair follicles expansion (P<0.01); the low dose and high dose of pomegranate peel polyphenols groups also could obviously decrease the degree of inflammation, keratinization, hair follicles expansion (P<0.05), the decreased degree in which was lower than medium dose of pomegranate peel polyphenols group and the control group (P<0.05). Conclusion The medium dose of pomegranate peel polyphenols group and the control group show the most significant anti-keratinization and anti-inflammatory effect on the rabbit ear acne model.关键词
石榴皮多酚软膏/抗角化/粉刺/组织病理学Key words
pomegranate peel polyphenols ointment/anti-keratinization/acne/histopathology分类
医药卫生引用本文复制引用
胡彬,杨逸璇,杨婉婷,王峰,陶梦颖,朱明芳..不同浓度的石榴皮多酚软膏对兔耳粉刺模型组织病理形态学的影响[J].湖南中医药大学学报,2018,38(4):397-401,5.基金项目
湖南省中医药科研基金资助项目(201425,201705) （201425,201705）
湖南中医药大学研究生创新项目(CX2016B379,2017CX18). （CX2016B379,2017CX18）
