江西农业大学学报2018，Vol.40Issue(2)：316-322,7.DOI:10.13836/j.jjau.2018041
热空气处理对靖安椪柑幼果柠檬酸代谢的影响
Effects of Hot Air Treatment on Citric Acid Metabolism In Jing'an Ponkan Fruitlet
摘要
Abstract
To explore the decomposition mechanism of citric acid in citrus fruit,Jing'an ponkan fruitlet were used to investigate the effects of hot air(40℃,48 h)treatment(HAT)on organic acid content and citric acid metabolism-related gene expression.The results showed that citric acid was the main organic acid in Jing'an ponkan fruitlet,and decreased significantly faster in HAT fruits than in the control.Meanwhile HAT could induce the expression of CitCS1 and CitCS2,which had significantly different levels in CitCS1 expressions.The expres-sion levels of CitAcos were also promoted by HAT.HAT could induce temporary CitIDH2 expression and promo-ted the expression of CitIDH3. In addition,HAT also significantly up-regulated CitGAD4 and CitGAD5 expres-sion,but had no significant effect on CitGS.These results suggested that HAT could promote the degradation of citric acid in Jing'an ponkan fruitlet,then the organic acid contents decreased,which was guided by the chan-ges of citric acid metabolism-related gene expression,and the up-regulation of CitAco1,CitIDH1,CitIDH3 and CitGAD4 expression maybe the major cause of organic acid decline in Jing'an ponkan fruit during maturation.关键词
热空气处理/靖安椪柑/柠檬酸/基因表达Key words
hot air treatment/Jing'an ponkan/citric acid/gene expression分类
农业科技引用本文复制引用
高阳,陈明,杨滢滢,阚超楠,陈楚英,刘善军,陈金印..热空气处理对靖安椪柑幼果柠檬酸代谢的影响[J].江西农业大学学报,2018,40(2):316-322,7.基金项目
国家自然科学基金项目(31360466)、江西省青年自然科学基金项目(20122BAB214024)和江西省教育厅项目(GJJ12223).Project supported by the National Natural Science Foundation of China(31360466),Jiangxi Province Youth Sci-ence Foundation(20122BAB214024)and Jiangxi Provincial Department of Education Project(GJJ12223) （31360466）
