临床骨科杂志2018，Vol.21Issue(2)：238-241,4.DOI:10.3969/j.issn.1008-0287.2018.02.044
MiR-490-3p靶向HMGA2在骨肉瘤细胞凋亡中的作用
MiR-490-3p regulates cell proliferation and apoptosis by targeting HMGA2 in osteosarcoma
摘要
Abstract
Objective Taking human osteosarcoma cell line MG-63 as the research object, the role and molecular mechanism of miR-490-3p inducing apoptosis of human osteosarcoma cell line MG-63 to study. Methods In this ex-periment,the miR-490-3p mimics group (control group) and adenovirus lenti-sh miR-490-3p group (drug group) were established respectively, with 6 reorients in each group.The inhibitory effect of miR-490-3p on the growth of cells was detected by four methyl azo blue(MTT) method. The effect of miR-490-3p on the apoptosis rate of MG-63 cells was detected under different experimental conditions by Annexin V-FITC/PI double staining method; the effect of miR-490-3p on the expression of HMGA2,Bcl-2,BAX and Caspase-3 protein was detected by Western blot. Re-sults MiR-490-3p by tacergeting HMGA2 could inhibit the proliferation of MG-63 cells,and the drug group had sta-tistical significance (P<0.05),compared with the control group. The percentage of cells with early apoptosis in the drug group was 58.90% ± 0.89%,which was significantly higher than that of the control group(5.34% ± 0.34%), both of which were statistically significant (P<0.05), HMGA2, Bcl-2, Bax, Caspase-3, Survivin protein expres-sion in MG-63 cells were defected by Western blot, the results showed that: after transfection with mir-490-3p, the expression of apoptotic protein Bax increased and the expression of anti-apoptotic protein Bcl-2 decreased,the ratio of Bax or Bcl-2 increased and the Caspase-3 protein of downstream mitochondrial apoptotic pathway was activated, the expression was reduced. Conclusions MiR-490-3p can significantly inhibit the proliferation of tumor cells and in-duce cell apoptosis,and its mechanism may be related to targeting HMGA2 up-regulated Bax/Bcl-2 protein expres-sion ratio,Caspase-3 protein activation cascade and regulation of Survivin protein expression.关键词
miR-490-3p/HMGA2/骨肉瘤细胞增殖/凋亡Key words
miR-490-3p/HMGA2/osteosareoma cells proliferation/apoptosis分类
医药卫生引用本文复制引用
刘伟,刘华强,许国星..MiR-490-3p靶向HMGA2在骨肉瘤细胞凋亡中的作用[J].临床骨科杂志,2018,21(2):238-241,4.基金项目
山东省潍坊市卫计委课题(编号:2016wsjs057) （编号:2016wsjs057）
