辽宁农业科学Issue(1)：24-29,6.DOI:10.3969/j.issn.1002-1728.2018.01.006
金针菇液体菌种培养条件优化及多因素交互影响
Study on Conditions Optimization and Multifactor Interaction Effects Using Design-expert for Flammulina velutiper Liquid Spawn
摘要
Abstract
Conditions optimization of Flammulina velutipe(Fr.) Sing liquid spawn were studied using Box-Be-hnken experimental design for response surface methodology in the paper.Based on single experiment,select-ed the main three factors (temperature, pH value and vulmn) were response surface methodology analyzed with design expert 8.0.6 design, and two multinomial mathematical model of Flammulina velutipe liquid spawn biomass were built.Under the condition of setting the inoculation amount of 10%and the speed of 160 r/min through single factor test,analysing significance and interaction of various factors,the results showed that the conditions optimization of Flammulina velutipe liquid spawn were temperature 25.96 ℃,medium capacity 106.73 ml,pH value 7.29.关键词
金针菇/菌丝体/培养基/响应面法Key words
Flammulina velutipe/Mycelium/Culture medium/Response surface methodology分类
农业科技引用本文复制引用
李超,李红..金针菇液体菌种培养条件优化及多因素交互影响[J].辽宁农业科学,2018,(1):24-29,6.基金项目
辽宁省公益基金项目(2015002012) （2015002012）
沈阳市重点科技研发项目(17-163-3-00) （17-163-3-00）
