辽宁农业科学Issue(2)：19-24,6.DOI:10.3969/j.issn.1002-1728.2018.02.004
葡萄霜霉病菌拮抗放线菌QH94发酵滤液的稳定性研究
Stability of the Ferment Filtrate of Antagonistic Actinomycetes Strain QH94 Against Plasmopara viticola
摘要
Abstract
The strain QH94 is an antagonistic actinomycetesagainst Plasmopara viticola.Studies on the stability of the fermentation filtratemade clear that the antifungal substance of strain QH94 belongs to extracellular sec-ondary metabolites.Inhibitory rate of fermentation filtrate was 86.44%~90.37%,when the temperature be-tween 20~100 ℃.When pH value was 2.0~5.0,inhibitory rate was above 90%.Antimicrobial activity was decreased after light treatment with time.But the stability to sunlight was better than UV light.Inhibitory rate of sunlight treatment decreased to 82.22% at most,while the UV light treatment was 80%.The filtrate had good storage tolerance.It can be stored for two months at 4 ℃,and one week at room temperature.The anti-fungal substance of fermentation filtrate was insoluble in petroleum ether,carbon tetrachloride,dichlorometh-ane,ethyl acetate,soluble in acetone and methanol,which showed the antifungal substance was water solu-ble.关键词
葡萄霜霉病菌/发酵滤液/抑菌活性/稳定性Key words
Plasmopara viticola/Fermentation filtrate/Antimicrobial activity/Stability分类
农业科技引用本文复制引用
蒋欣陶,梁春浩,臧超群..葡萄霜霉病菌拮抗放线菌QH94发酵滤液的稳定性研究[J].辽宁农业科学,2018,(2):19-24,6.基金项目
国家重点研发技术自助项目(2017YFD0201104) （2017YFD0201104）
辽宁省博士启动基金项目(20170520173) （20170520173）
