中国海洋大学学报（自然科学版）2018，Vol.48Issue(4)：66-72,7.DOI:10.16441/j.cnki.hdxb.20170072
虾夷扇贝SOXH不同剪接形式的鉴定和表达分析
Identification and Expression Profiling of Different Splicing Patterns of SOXH in Scallop Patinopecten yessoensis
摘要
Abstract
SOXH,a member of SOX gene family,plays key roles in spermatogonium differentiation and spermatogenesis in vertebrates.In recent years,research shows SOXH is not vertebrate specific,but it remains unclear in invertebrates regarding how SOXH works and what its function is.In this study,SOXH was successfully identified from the Yesso scallop genome and transcriptome by bioinformatics analysis.Three splicing patterns of SOXH were found,coding 694,679,1051 amino acids,respectively.Expression profiles of different splicing forms in scallop ovary and testis of four developmental stages were obtained by qRT-PCR,which showed that SOXH predominantly expressed in mature testis and lowly expressed in ovary.Additionally,expression of SOXH in mature testis prefer to the third splicing pattern.The study also showed that the three splicing forms presented different expression patterns in early development.All these results revealed that SOXH played important roles in scallop spermatogenesis and embryonic/larval development,but the three splicing forms of SOXH may exert diverse functions.This study providesa base for further research on SOXH function and its molecular mechanism in invertebrates.关键词
SOXH/剪接形式/虾夷扇贝Key words
SOXH/splicing pattern/Patinopecten yessoensis分类
生物科学引用本文复制引用
于佳辰,张玲玲,李仰平,李若佼,李婉茹,孙文倩,包振民..虾夷扇贝SOXH不同剪接形式的鉴定和表达分析[J].中国海洋大学学报（自然科学版）,2018,48(4):66-72,7.基金项目
国家自然科学基金项目(31572600) （31572600）
青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2016ASKJ14) （2016ASKJ14）
中央高校基本科研业务费专项资金项目(201564009)资助 Supported by the National Natural Science Foundation of China (31572600),the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology (2016ASKJ14),and the Fundamental Research Funds for the Central Universities (201564009) （201564009）
