山东医药2018，Vol.58Issue(16)：9-12,4.DOI:10.3969/j.issn.1002-266X.2018.16.003
葛根素对MC3T3-E1成骨前体细胞增殖能力的影响及其机制
Effect of puerarin on proliferation ability of osteoblast MC3T3-E1 cells
摘要
Abstract
Objective To investigate the effect of puerarin on the proliferation ability of osteoblast MC 3T3-E1 and its possible mechanism.Methods MC3T3-E1 cells in the logarithmic growth were selected and treated with different concen-trations of puerarin at different time.CCK-8 was used to detect the effect on cell proliferation.The results showed that the cell proliferation ability was the highest when the cells were treated with 0.1 μmol/L puerarin for 48 h.The Mc3t3-e1 cells were randomly divided into the blank control group, the puerarin group, and the estrogen group, respectively, and were added with the complete medium of α-MEM,0.1 μmol/L puerarin,and 0.01 μmol/L estrogen.After 48 h,we observed cell autophagy under electron microscope.Western blotting was used to detect phosphate-activated mitogen protein kinase (p-AMPK),microtubule associated protein 1 light chain 3(LC3)-Ⅱ/LC3Ⅰexpression.The effects of interfering AMPK on cell autophagy and LC3-Ⅱ/LC3-Ⅰexpression of MC3T3 E1 treated with puerarin.Results The cell organelles,nuclei and chromosome were normal in the blank control group.The cytoplasm of puerarin and oestrogens showed a large number of annular structures,and the substance had bilayer membrane structure specific to autophagy.The expression of p-AMPK and LC3-Ⅱ/LC3-Ⅰin the puerarin group and estrogen group was significantly higher than that of blank control group,and the estrogen group was significantly higher than the puerarin group(all P<0.01).Interfering AMPK expression inhibited puera-rin-mediated MC3T3-E1 cell autophagy,and decreased the LC3-Ⅱ/LC3-Ⅰexpression(all P<0.01).Conclusion Puerarin can enhance the proliferation of MC3T3-E1 cells and the activation of AMPK/LC3 signaling pathway-mediated autophagy is one of the possible mechanisms.关键词
骨质疏松症/葛根素/丝裂原活化蛋白激酶/微管相关蛋白1轻链3/成骨前体细胞/自噬/细胞增殖Key words
osteoporosis/puerarin/mitogen-activated protein kinase/microtubule-associated protein 1 light chain 3/osteoblast precursor cell/autophagy/cell proliferation分类
医药卫生引用本文复制引用
冯倩,俞鹏,程嵩奕,曾祥伟,杨瑞,杨婧,赵凤鸣,朱家鹏,詹秀琴..葛根素对MC3T3-E1成骨前体细胞增殖能力的影响及其机制[J].山东医药,2018,58(16):9-12,4.基金项目
江苏省自然科学基金面上项目(BK20131417) （BK20131417）
江苏高校优势学科建设工程资助项目(中西医结合). （中西医结合）
