山东医药2018，Vol.58Issue(16)：17-20,4.DOI:10.3969/j.issn.1002-266X.2018.16.005
淫羊藿苷对MC3 T3-E1成骨前体细胞的成骨诱导作用及意义
Osteoinductivity of icariin on MC3T3-E1 cells
摘要
Abstract
Objective To evaluate cytotoxicity and osteoinductivity of icarrin on MC 3T3-E1 osteoblast precursor cells, which provides basis for bone tissue regeneration.Methods The MC3T3-E1 cells were randomly divided into the observa-tion group,the negative control group,and the positive group.The cells in the observation group were cultured with 100μL of 1×10-6,1×10-5,1×10-4,1×10-3,and 1×10-2mmol/L icarrin,respectively;cells in the negative control group were added with the same volume of DMSO and cells in the positive group with the same volume of rhBMP-2.Subsequently,the proliferation rate on the 3rd and 7th day was tested with MTT method.The cell survival rate was determined by dead/living cell fluorescence detection.The calcium nodules were observed by Alizarin red calcium nodules stain.The calcium con-tent,alkaline phosphatase(ALP),and osteocalcin were tested by cellular ultrasonic cracking.Results The cell prolifer-ation rate in the observation group with 1×10-2mmol/L icariin was lower than that in the positive and negative groups on the 3rd day(both P<0.05), and the cell proliferation rate in the observation group with 1×10-2mmol/L and 1×10-3 mmol/L icariin was lower than that in the positive and negative groups on the 7th day(both P<0.05).The cell survival rate in the observation group with 1×10-3mmol/L icariin was lower than that in the positive group(P<0.05).The cell survival rate in the observation group with 1×10-2mmol/L icariin was lower than that in the positive and negative groups (both P<0.05).No calcium nodules formed in the negative group and a large area of calcium nodules was found in the positive group.In the observation group,the calcium nodule gradually increased with the increasing concentrations of icari -in.The ALP activity and osteocalcin in the observation group were higher than those in the negative group(all P<0.05). The Ca content in each of the observation group with 1×10-4,1×10-3,and 1×10-2mmol/L icariin was higher than that in the negative group,and the Ca content in each of the observation group with 1×10-6,and 1×10-5mmol/L icariin was low-er than that of the positive group(all P<0.05).Conclusion 10-4mmol/L icariin, with lower cytotoxicity and osteoin-ductivity,which is similar to rhBMP-2,can be used as an alternative agent of growth factor combined with bone repair ma-terials to promote the bone tissue regeneration.关键词
淫羊藿苷/成骨前体细胞/细胞毒性/骨缺损/成骨诱导/骨修复材料Key words
icariin/osteblast precursor cell/cytotoxicity/bone defect/osteoinductivity/bone repair materials分类
医药卫生引用本文复制引用
王建茹,张育敏,韩波..淫羊藿苷对MC3 T3-E1成骨前体细胞的成骨诱导作用及意义[J].山东医药,2018,58(16):17-20,4.基金项目
山西省自然科学基金资助项目(201601D011126) （201601D011126）
山西中医学院博士启动基金项目(2014bk05) （2014bk05）
山西省中医药科研课题(2016ZYYC02). （2016ZYYC02）
