山东医药2018，Vol.58Issue(16)：21-25,5.DOI:10.3969/j.issn.1002-266X.2018.16.006
XACB/MSCs/Lv-GFP-FGF-2的制备及其对兔激素性股骨头缺血坏死的治疗效果观察
Therapeutic effect of XACB/MSCs/Lv-GFP-FGF-2 complex on steroid-induced avascular necrosis of femoral head in rabbits
摘要
Abstract
Objective To prepare complex of lentivirus-mediated fibroblast growth factor 2(FGF-2)gene which was used to transfect into bone marrow stromal stem cells(MSCs)and then were co-cultured with xenogeneic antigenic cancel-lous bone(XACB),and to observe its therapeutic effect on steroid-induced avascular necrosis of femoral head and its effect on the expression of TNF-αin femoral head tissues.Methods Rabbit MSCs were isolated and cultured in vitro.The lenti-virus-mediated FGF-2 gene was used to transfect the MSCs and then were cultured with XACB to obtain tissue engineering bone of XACB/MSCs/Lv-GFP-FGF-2 complex.Sixty rabbit models of steroid-induced avascular necrosis of femoral head which were established by injection of endotoxin and methylprednisolone were randomly divided into 5 groups with 12 in each group.Core decompression and bone grafting were performed.In group A, bone grafting was not performed,autolo-gous cancellous bone was implanted in the group B, XACB/MSCs were implanted in the group C, XACB/MSCs/Lv-GFP was implanted in the group D,and XACB/MSCs/Lv-GFP-FGF-2 was implanted in the group E.Four rats were executed at 3,6,12 weeks after operation in each group,then we observed the general appearance of femoral head.The positive ex-pression rate of TNF-αwas detected by immunohistochemistry.The real-time PCR was used to detect the expression of TNF-αmRNA in the femoral head tissues.Results Three weeks after operation, a small amount of new bone formation were found in the group E,there were no new bone formation in the other groups.At 6 weeks after operation,the defect ar-eas in the group A were filled with granulation tissue and there were no new bones.The defect areas in the group B,C and D were still visible and the new bones were visible at the edge.Most of the defect areas in the group E were filled with new bones,and bone graft was basically absorbed.At 12 weeks after operation,only a small amount of new bone was formed in the group A,and defect areas of the groups B,C,and D were not repaired completely.The defect in the group E was re-stored to normal cancellous bone.At 3,6,and 12 weeks after operation,the positive expression rate of TNF-αand relative expression of mRNA in the femoral head tissues of the groups B,C,D,and E were decreased gradually(all P<0.05). At 3 weeks after operation,the positive expression of TNF-αand the mRNA relative expression of femoral head in the group E were lower than those in the groups A,B,C,and D(all P<0.05).At 6,12 weeks after surgery,the TNF-αpositive expression rate and mRNA relative expression level of the femoral bone tissue at the same time point were in the following order:group A>group B >group C, group D >group E(all P <0.05).Conclusion The tissue engineering bone XACB/MSCs/Lv-GFP-FGF-2 complex is successfully prepared,and it has a good effect on the treatment of steroid-induced avascular necrosis of the femoral head in rabbits by inhibiting the expression of TNF -αin the femoral head tissues.关键词
股骨头缺血坏死/组织工程骨/异种脱抗原松质骨/骨髓基质干细胞/肿瘤坏死因子α/兔Key words
avascular necrosis of the femoral head/tissue engineering bone/autologous cancellous bone/bone mar-row stromal cells/basic fibroblast growth factor/tumor necrosis factor-α/rabbits分类
医药卫生引用本文复制引用
王健波,彭吾训,张健,张飞,张槐,赵胤,李青,邓进..XACB/MSCs/Lv-GFP-FGF-2的制备及其对兔激素性股骨头缺血坏死的治疗效果观察[J].山东医药,2018,58(16):21-25,5.基金项目
贵阳市科技局基金资助项目(GY2016-3). （GY2016-3）
