上海农业学报2018，Vol.34Issue(1)：74-78,5.DOI:10.15955/j.issn1000-3924.2018.01.14
基于线粒体细胞色素b基因的崇明岛白山羊系统进化研究
Phylogenetic research on Chongming Island's white goats based on mitochondrial cytochrome b genes
摘要
Abstract
The 72 samples of white goats were collected from Chongrning Island and Shanghai surrounding areas and the PCR amplification and sequencing of their mitochondrial cytochrome b (cyt b) genes were made through designing two pairs of primers (CF1/CF2,MA1/MA2) according to the mitochondrial genome sequences (NC_005044) of Capra hircus.The results showed that the length of their mitochondrial cyt b genes is 1 140 bp.The phylogenetic analysis showed that the 72 samples' mitochondrial cyt b genes can be divided into 2 major populations,the 32 samples belong to the Boer goat population (BOR),and the rest are the Yangtze River Delta white goat population (YRD)whose cyt b sequences have been recorded in the Genbank (KY348291-KY348330).The research indicated that there are a large number of hybrids in the white goat population of Chongming Island,but 6 samples from 2 sampling sites of Chongrning Island are independently clustered together,meaning that the 6 samples may be a local white goat population of Chongming Island.The statistic analysis found that there are 8 SNP loci in the mitochondrial cyt b genes of both those 6 samples and the other YRD population.So Chongming white goats on the 2 sampling sites of Chongming Island should be protected and further investigated.关键词
白山羊/线粒体基因组/系统进化Key words
White goat/Mitochondrial genome/Phylogeny分类
农业科技引用本文复制引用
高骏,吕玉华,戴建军,张德福,易建中,李红,刘成倩,孙凤萍..基于线粒体细胞色素b基因的崇明岛白山羊系统进化研究[J].上海农业学报,2018,34(1):74-78,5.基金项目
上海市市级农口系统青年人才成长计划[沪农青字(2015)第1-36号] （2015）
上海市科委创新行动计划[15140900300] （）
