上海农业学报2018，Vol.34Issue(1)：79-83,5.DOI:10.15955/j.issn1000-3924.2018.01.15
猪流行性腹泻病毒流行株M基因的克隆与序列分析
Clone and sequence analysis of M gene of field porcine epidemic diarrhea virus
摘要
Abstract
The M genes of 2 clinical isolates of porcine epidemic diarrhea virus(PEDV),D1 and D3 were cloned by RT-PCR,then their nucleotide and encoding amino acid sequences were analyzed.The results showed that the nucleotide sequence homology of the 2 isolates was 98.1％ and the homology of amino acid sequence was 97.8％.The phylogenetic relationship of the isolate D1 and the reference strain EAS1,SM98,CHM2013 isolates were relatively close,while the phylogenetic relationship of the isolate D3 and PEDV-LY,PEDV-WS,CH22 isolates were relatively close.Compared with the reference strain CV777,the location and sequence composition of 4 antigen determinants in M protein of isolate D1 were changed.关键词
猪流行性腹泻病毒/流行毒株/M基因Key words
Porcine epidemic diarrhea virus/epidemic strain/M gene分类
农业科技引用本文复制引用
丁卫星,王荣谈,李春华,夏叶,郭佳宏,倪建平,缪德年,赵敏..猪流行性腹泻病毒流行株M基因的克隆与序列分析[J].上海农业学报,2018,34(1):79-83,5.基金项目
上海市科技支撑项目(13391901500) （13391901500）
上海工程技术研究中心建设专项(16dz2250700) （16dz2250700）
