色谱2018，Vol.36Issue(4)：356-361,6.DOI:10.3724/SP.J.1123.2017.10035
全自动固相萃取-超高效液相色谱-串联质谱法测定婴幼儿湿疹类产品中42种糖皮质激素
Determination of 42 glucocorticoids in infant eczema products by auto-solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method was developed for the determination of 42 glucocorticoids in infant eczema products by auto-solid phase extraction-high performance liquid chromatography-tandem mass spectrometry(ASPE-UHPLC-MS/MS). The analytes were extracted by acetonitrile after disper-sing in saturated sodium chloride solution. Macromolecules matrix were precipitated by adding potassium and zinc acetate,and then purified by HLB SPE column. The chromatographic sepa-rations were performed on a DIKMA EndeavorsilTMC18 column(100 mm×2.1 mm,1.7 μm). Acetonitrile and 0.1%(v/v)formic acid aqueous solution were used as the mobile phases. Electrospray ionization(ESI)source was operated in the positive mode using multiple reaction monitoring(MRM)scanning mode. The results were quantified by external standard method. The correlation coefficients of linear calibration curves were greater than 0.998 in the corre-sponding mass concentration ranges. The average recoveries at three spiked levels ranged from 78.7% to 101.5%,and the relative standard deviations(RSDs)were 2.0%-8.8%(n=6). The LODs(S/N≥3)and LOQs were 0.002-0.031 mg/kg and 0.000 7-0.103 mg/kg,respectively. The method is simple,rapid,sensitive,and reliable,and can be used for the detection of glucocorticoids in infant eczema products.关键词
超高效液相色谱-串联质谱/自动固相萃取/糖皮质激素/婴幼儿湿疹产品Key words
ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS-MS)/auto-solid phase extraction(ASPE)/glucocorticoid/infant eczema products分类
化学化工引用本文复制引用
潘小红,殷帅,刘玉玲,龙凌云,赵薇,孟庆玉..全自动固相萃取-超高效液相色谱-串联质谱法测定婴幼儿湿疹类产品中42种糖皮质激素[J].色谱,2018,36(4):356-361,6.基金项目
湖南省自然科学基金(2016JJ6079).Natural Science Foundation of Hunan Province(No.2016JJ6079). （2016JJ6079）
