色谱2018，Vol.36Issue(4)：400-407,8.DOI:10.3724/SP.J.1123.2017.11003
超高效液相色谱-串联质谱法同时检测动物组织中15种保水功能药物
Simultaneous determination of 15 water-holding functional drugs in animal tissues using ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method based on ultra performance liquid chromatography-tandem mass spec-trometry(UPLC-MS/MS)was developed for the determination of 15 water-holding functional drugs in animal tissues. The analytes were extracted with acetonitrile containing 1.0%(v/v) methanol,purified by Oasis PRiME HLB SPE column,and analyzed by Acquity UPLC BEH C18 column(50 mm×2.1 mm,1.7 μm)using methanol and 0.1%(v/v)formic acid aqueous solu-tion as the mobile phases. The analytes were detected using an electrospray ionization(ESI) source under the MRM mode. The calibration curves of the analytes were linear in the range of 1.0-50.0 μg/kg(r≥0.994 9),and the limits of quantification were all less than 1.0 μg/kg in animal tissues. The recoveries of the 15 water-holding functional drugs ranged from 60.0%-111.0%in animal tissue samples,with the intra and inter RSDs of 0.56%-11.5% and 2.31%-14.8%,respectively. The method can meet the requirements for the determination of the drug residues in animal tissues. It provides a new idea to identify potential hazards in animal-derived foods and to monitor illegal addition.关键词
超高效液相色谱-串联质谱/残留/保水功能药物/动物组织Key words
ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)/residues/water-holding functional drugs/animal tissues分类
化学化工引用本文复制引用
刘洪斌,姚喜梅,田亚平,张鹭,蔡英华,姜艳彬,李颖..超高效液相色谱-串联质谱法同时检测动物组织中15种保水功能药物[J].色谱,2018,36(4):400-407,8.基金项目
"十二五"国家科技支撑重点项目(2014BAD13B05).Key Project in the National Science＆Technology Pillar Program during the Twelfth Five‐Year Plan Period(No.2014BAD13B05)． （2014BAD13B05）
