色谱2018，Vol.36Issue(4)：408-412,5.DOI:10.3724/SP.J.1123.2017.11002
超高效液相色谱-串联质谱法测定阿胶中马、牛、羊、猪、骆驼、鹿皮源成分
Determination of the ingredients of horse,ox,sheep,pig,camel and deer skin in colla corii asini by ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method was established for detecting the ingredients of horse,ox,sheep,pig, camel and deer skin in colla corii asini using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Theoretical marker peptides were selected by comparing the collagen sequences in the skins of donkeys,horses,oxen,sheep,pigs,camels,and deer. Marker peptides were identified by protease cutting techniques and high resolution mass spec-trometry and then analyzed by UPLC-MS/MS. The separation was performed on a UPLC system with a BEH C18 column via the gradient elution of acetonitrile containing 0.1%(v/v)acetic acid and water containing 0.1%(v/v)acetic acid. The marker peptides were identified in the modes of electrospray positive ionization(ESI+)and multiple reaction monitoring(MRM). The marker peptides for the horse,ox,sheep,pig and camel skin were detected in 15 samples. The method is simple,highly reproducible,and suitable for the identification of mixed skin ingredi-ents in colla corii asini. It has been successfully used to detect the authenticity of colla corii asini.关键词
超高效液相色谱-串联质谱/特征肽/胶原蛋白/阿胶Key words
ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)/marker peptide/collagen/colla corii asini分类
化学化工引用本文复制引用
杭宝建,田晨颖,陈晓,邢晟,石峰,冷佳蔚,巩丽萍..超高效液相色谱-串联质谱法测定阿胶中马、牛、羊、猪、骆驼、鹿皮源成分[J].色谱,2018,36(4):408-412,5.基金项目
国家重大新药创制专项(2014ZX0930437).Major New Drug Creation Program of China(No.2014ZX0930437)． （2014ZX0930437）
