实用肝脏病杂志2018，Vol.21Issue(3)：393-396,4.DOI:10.3969/j.issn.1672-5069.2018.03.019
敲减CCAAT/增强子结合蛋白α通过增加O-GlcNAc糖基化水平促进肝癌细胞体外增殖
Downregulation of CCAAT/ enhancer-binding protein alpha promotes growth of hepatocellular carcinoma cells in vitro by increasing O-GlcNAcylation
摘要
Abstract
Objective To explore the roles and potential mechanism of CCAAT/enhancer-binding protein alpha(C/EBPα)in the growth of hepatocellular carcinoma(HCC)cells in vitro. Methods The Hep3B cells were transfected with RNAi lentivirous,and the mRNA and protein of C/EBPα were detected by qRT-PCR and Western blot respectively. The growth of HCC cells in normal glucose(NG,4.5 g/L)and low glucose(LG,1 g/L) were assessed by CCK-8 assay and quantified based on the absorbance value at OD450. The protein levels of O-GlcNAcase(OGA),O-GlcNAc transferase(OGT)and the whole O-GlcNAcylation in normal glucose and low glucose were detected by Western blot. Results The C/EBPαmRNA level was down-regulated by(7.5±2.3)folds (P<0.05)and its protein was decreased by(8.8±0.25)folds(P<0.001)in transfected with RNAi lentivirous cells (sh-C/EBPα)compared with control cell(sh-Con). Knocking down C/EBPα promoted the growth of HCC cells in vitro. The growth rates of sh-C/EBPα cells increased by 15.4%(P<0.05)and 25.0%(P<0.01)in low glucose (LG,1 g/L)condition at 48 hours and 72 hours;The protein of OGA decreased by 70.1%(P<0.01),51.4%(P<0.05)and 6 1.2%(P<0.05)in normal gulucose,low glucose at 24 h and 48 h. Whole O-GlcNAcylation was up-regulated by 80.6% in the C/EBPα knocked-down cells in low glucose at 48 h. Conclusions The downregulation of CCAAT/enhancer-binding protein alpha promotes the growth of hepatocellular carcinoma cells in vitro likely by increasing O-GlcNAcylation in some extent.关键词
肝细胞癌/CCAAT/增强子结合蛋白α/O-GlcNAc糖基化/体外Key words
Hepatoma/CCAAT/enhancer-binding protein alpha/O-GlcNAcylation/In vitro引用本文复制引用
李文姣,曹海超,马澜婧,张松,丁美玲,张浩浩,聂勇战..敲减CCAAT/增强子结合蛋白α通过增加O-GlcNAc糖基化水平促进肝癌细胞体外增殖[J].实用肝脏病杂志,2018,21(3):393-396,4.基金项目
国家杰出青年科研基金资助项目(编号:81225003) （编号:81225003）
