实用肝脏病杂志2018，Vol.21Issue(3)：397-400,4.DOI:10.3969/j.issn.1672-5069.2018.03.020
c-Met基因过表达脐带间充质干细胞株的构建
Construction of overexpression of c-met in human umbilical cord mesenchymal stem cells with lenti-vector transfection in vitro
摘要
Abstract
Objective To construct the overexpression of c-met in human umbilical cord mesenchymal stem cells(hUMSCs)with lenti-vector transfection in vitro. Methods The phenotype of hUMSCs was determined by Flow cytometry analysis,and the hUMSCs were transfected by different multiple of infection(MOI with c-met lenti-vector. The optimal MOI was determined under fluorescence microscopy. The c-Met protein was detected by Western blot. Results P5 gegeration of hUMSCs were CD44/CD90/CD105-positive,and CD31/CD45/CD34-negative,indicating the hUMSCs were obtained with specific surface markers. The optimal MOI was 80,and after puromycin screening,the transfected efficiency was up to 100%. The c-Met protein was overexpressed in the transfected hUMSCs by Western blot detection. Conclusion c-Met overexpressing hUMSCs were successfully constructed,which might be beneficial for further study.关键词
人脐带间充质干细胞/c-Met基因/转染/体外Key words
Human umbilical cord mesenchymal stem cells/c-Met/Transduction/In vitro引用本文复制引用
张永婷,朱传龙,李军,章莉莉..c-Met基因过表达脐带间充质干细胞株的构建[J].实用肝脏病杂志,2018,21(3):397-400,4.基金项目
国家自然科学基金资助项目(编号:81770591) （编号:81770591）
江苏省高层次卫生人才"六个一工程"资助项目(编号:LGY2016005) （编号:LGY2016005）
