生态环境学报2018,Vol.27Issue(4):730-735,6.DOI:10.16258/j.cnki.1674-5906.2018.04.019
极干旱区大气PM2.5对质粒DNA的氧化性损伤研究
Analysis of Oxidative Capacity of PM2.5in Arid Area
摘要
Abstract
In recent years, the rapid economic development of Hotan City and rapid population growth caused increase in air pollution from vehicles and industry. In order to evaluate the effect of dust whether on the toxicity of PM2.5of urban area, the PM2.5 samples were collected from January to December 2014 to analyze the oxidative capacity of the PM2.5particles by plasmid scission assay. The result showed that, the mass concentration of PM2.5ranged from 70 to 2 489 μg·m-3and which had been increased with the wind speed. The TD30(toxic dose of PM causing 30% of plasmid DNA damage, μg·mL-1) was used to indicate the oxidative capacity. The TD30values of whole and water soluble fraction of PM2.5samples were 444~27 480 μg·mL-1and 481~20 434 μg·mL-1, respectively. Both of the whole and water soluble fractions indicated that the lower the wind speed caused the higher the oxidative damage to plasmid DNA. During sand and non-sand period, the average TD30values of whole and water soluble fraction were 9 464 μg·mL-1and 8 008 μg·mL-1and water soluble were 5 494 μg·mL-1and 7 822 μg·mL-1respectively. The oxidative damage of whole particles were higher than their corresponding water-soluble fractions during the non-dust storm period, on contrast, the toxicity of the particles was mainly sourced from their water soluble fraction during the dust storm period. Both of the TD30in whole and water soluble fraction positively correlated with PM2.5mass concentration indicated that the mass concentration of PM2.5played an important role in oxidative damage.关键词
PM2.5/氧化性损伤/质粒DNA评价法/和田市城区Key words
PM2.5/oxidative damage/plasmid DNA/Hotan City分类
资源环境引用本文复制引用
图尔贡·艾尔肯,迪丽努尔·塔力甫,邵龙义,买丽克扎提·买合木提,亚力昆江·吐尔逊,阿布力克木·阿布力孜..极干旱区大气PM2.5对质粒DNA的氧化性损伤研究[J].生态环境学报,2018,27(4):730-735,6.基金项目
国家自然科学基金项目(41465007) (41465007)
中国沙漠气象科学研究基金项目(Sqj2013003) (Sqj2013003)