西南国防医药2018，Vol.28Issue(4)：304-307,4.DOI:10.3969/j.issn.1004-0188.2018.04.002
紫外线和热力对55型腺病毒的灭活效果
Inactivation effect of UV and heat on adenovirus 55
摘要
Abstract
Objective To explore the inactivation effects of UV and heat on human Adenovirus 55 (HAdV-55) and provide references for prevention and control of HAdV-55 infection.Methods HAdV-55 was placed under 245 nm UV and 65℃ heat for 30 min,1 h and 2 h,respectively.Quantitative PCR and virus titer test were performed to examine the impacts of various factors on HAdV-55-infected HEp-2 cells.Results After 30 min's UV action,the level of HAdV-55-infected HEp-2 cells decreased by 50％ approximately;after 1 h and 2 h's UV action,the virus was still infectious to some extent,but the level of infection decreased greatly.After 30 min's heat treatment at 65 ℃,the level of HAdV-55-infected HEp-2 cells decreased by about 104 orders of magnitude.Virus inactivated by UV and heat showed a significantly lowered capability of generating progeny virus.Conclusion Both UV and Heat can inactivate HAdV-55.The effect of inactivation depends on the time of action.More than 30 min's 254 nm UV action and heat treatment at 65℃ can considerably reduce the capability of HAdV-55 to generate progeny virus.关键词
紫外线/热力/灭活/腺病毒Key words
UV/heat/inactivation/HAdV分类
医药卫生引用本文复制引用
刘媛,王文博,邹自英,邱薇,范泉水,冯子良,熊杰..紫外线和热力对55型腺病毒的灭活效果[J].西南国防医药,2018,28(4):304-307,4.基金项目
国家自然科学基金青年项目(81301445) （81301445）
全军医学科技青年培育项目(14QNP057) （14QNP057）
上海市医学生物防护重点实验室开放课题(SKLM1401) （SKLM1401）
