眼科新进展2018，Vol.38Issue(4)：339-343,5.DOI:10.13389/j.cnki.rao.2018.0079
白细胞介素-34在大鼠角膜移植排斥反应中的作用机制
Mechanism of IL-34 in rat corneal allograft rejection
摘要
Abstract
Objective To investigate the expression of interleukin-34 (IL-34) after corneal transplantation in rats and its mechanisms of action in immune rejection.Methods SD rats were used as donors and Wistar rats as the recipients to establish corneal transplantation experimental models.Together 60 Wistar rats were randomly divided into 3 groups according to the random number table methods,and rats in B group was transplantated with autologous cornea,while C and D groups received allogeneic corneal transplantation.Another 10 rats were collected as normal controls (A group).After B,C group was treated with postoperative Tarivid eye drops,D group was treated with TobraDex eye drops postoperatively.The survival rate of corneal grafts and survival analysis were evaluated in the 10 rats from the 4 groups,and then the corneal grafts were taken from the rest of rats at the fourteenth day after the operation for hstopathological,immunohistochemical and RT-PCR examinations.Results Survival analysis showed that immune rejection did not occur in A and B group,and the mean survival time (MST) was (26.00 ± 0.97) days in D group,which was much higher than that in group C[(10 ± 1.55) days] (P ＜0.001).HE staining showed that there was obvious inflammatory cell infiltration and neovascularization in the corneal tissue of C group,and there were only a few inflammatory cells and neovascularization in D group.Immunohistochemistry showed that IL-34 protein expression in C group (0.089 4 ± 0.005 6) was significantly higher than that of A group (0.037 7 ± 0.002 3),B group (0.068 4 ±0.004 4) and group D (0.044 5 ± 0.004 5) (F =145.21,P ＜ 0.01),and its expression was mainly located in the epithelial and stromal layer.RT-PCR showed that the expression levels of IL-34,IL-1β,IL-17A,TNF-α mRNA in corneal tissue of C group were significantly higher than those in A,B and D group (all P ＜ 0.05).Conclusion IL-34 may involve in the rejection after corneal transplantation in rats,and TobraDex eye drops can delay the rejection by inhibiting the expression of IL-34 and related signaling pathways.关键词
白细胞介素-34/免疫排斥/角膜移植Key words
IL-34/immune rejection/corneal transplantation分类
医药卫生引用本文复制引用
卢晓丽,吴京,马明,巫小送,陈林江,文静,于健..白细胞介素-34在大鼠角膜移植排斥反应中的作用机制[J].眼科新进展,2018,38(4):339-343,5.基金项目
广州市科技计划项目(编号:2017A020211005、201607010386) （编号:2017A020211005、201607010386）
南方医院院长基金(编号:2014B014、2014C005) Science and Technology Planning Project of Guangzhou Province (No:2017A020211005,201607010386) （编号:2014B014、2014C005）
Presidents fund of Nanfang Hospital (No:2014B014,2014C005) （No:2014B014,2014C005）
