应用生态学报2018，Vol.29Issue(4)：1190-1196,7.DOI:10.13287/j.1001-9332.201804.040
链格孢菌粗毒素对菊花‘神马’幼苗生长及生理代谢的影响
Effects of crude toxin from Alternaria alternata on the growth and physiological metabolism of chrysanthemum 'Jinba' seedlings
摘要
Abstract
The chrysanthemum black spot caused by Alternaria alternata significantly reduced the quality and yield of chrysanthemum.The crude toxin secreted by A.alternata in the metabolic process have elopathic effects on plants,which is the main pathogenic factor for the occurrence of chrysanthemum black spot.The pathogenic fungi A.alternate was isolated from chrysanthemum black spot leaves,The effects of crude toxin on plant height,stem diameter,root length,resistant material content,membrane relative permeability,polyphenol oxidase (PPO),peroxidase (POD),phenylalanine ammonia-lyase (PAL) in different treatments of chrysanthemum'Jinba'seedlings were investigated.The results showed that the crude toxin of A.alternata had an inhibition effect on plant height,stem diameter and root length.The toxin concentration was positively correlated with the inhibitory effect.14 days after crude toxin treatment,plant height,stem diameter and root length were significantly inhibited,with an reduction of 28.9％,21.4％ and 23.3％,respectively.The cell membrane permeability of leaf increased with the toxin concentration.Under the same toxin concentration,the cell membrane permeability first increased and then decreased with the treatment duration.The contents of soluble protein,malondialdehyde (MDA) and proline in leaves were significantly increased after treatment with the toxin solution.The increases of PAL,POD and PPO were the most significant in 10 times A.alternatacrude toxin treatment.The pathogenicity of A.alternate crude toxin to the chrysanthemum'Jinba'seedlings was mainly through inhibiting the normal growth of roots and stems,destructing the root cell membrane permeability and increasing the contents of MDA,normal soluble sugar and proline,and promoting the activities of PAL,POD,PPO in leaf tissues.关键词
菊花/链格孢菌/毒素/细胞膜透性/脯氨酸Key words
chrysanthemum/Alternaria alternate/toxin/cell membrane permeability/proline引用本文复制引用
张凯凯,赵爽,陈慧杰,倪嘉琪,姜晓帆,陈发棣,房伟民..链格孢菌粗毒素对菊花‘神马’幼苗生长及生理代谢的影响[J].应用生态学报,2018,29(4):1190-1196,7.基金项目
本文由江苏省产学研前瞻性联合研究项目(BY2016077-06)、江苏省农业三新工程项目(SXGC2017280)、江苏省农业科技自主创新资金项目(CX142023)和中央高校基本科研业务费专项(KYCYL201501)资助 （BY2016077-06）
This work was supported by the Prospective Production and Research Joint Research Project of Jiangsu Province,China (BY2016077-06),the Three New Agricultural Projects of Jiangsu Province,China(SXGC2017280),the Agricultural Science and Technology Independent Innovation Foundation of Jiangsu Province,China (CX142023) and the Basic Scientific Research Service Fee for Central Colleges and Universities (KYCYL201501). （BY2016077-06）
