中国动脉硬化杂志2018，Vol.26Issue(3)：217-221,5.
Ezrin基因沉默通过降低Calpain-1活性抑制血管紧张素Ⅱ诱导的ABCA1降解
Ezrin silencing inhibits angiotensin Ⅱ-induced ABCA1 decrease by reducing calpain-1 activity
摘要
Abstract
Aim To investigate the effects of Ezrin gene knockout on calpain-1 activity and adenosine triphosphate binding cassette transporter A1 (ABCA1) protein levels in RAW264.7 cells.Methods RAW264.7 cells transfected with small interfering RNA (siRNA) targeting Ezrin were subject to real time PCR and Western blot to detect the inhibition ratio.Added with angiotension Ⅱ (Ang Ⅱ) or Ang Ⅱ + Ezrin siRNA,calpain-1 protein expression and activity were determined by using Western blot.Then,the expression of ABCA1 protein was examined by Western blot after the treatment with Ang Ⅱ,Ezrin siRNA,calpain inhibitor N-acetyl-L-leucyl-L-leucyl-L-norleucine (ALLN).Results Ezrin mRNA and protein expression in Ezrin siRNA groups were inhibited(P＜0.05).The calpain-1 expression were upregulated by Ang Ⅱ treatment (4.92±0.23 vs 1.00±0.17,P＜0.05),which were reversed by Ezrin siRNA (0.23±0.21 vs 4.92±0.23,P＜0.05).Therefore,the ABCA1 protein expression was inhibited by addition of Ang Ⅱ (0.167±0.055 vs 0.732±0.072),which were reversed by Ezrin siRNA (0.611±0.048 vs 0.167±0.055,P＜0.05).There were no statistically significant differences at ABCA1 protein levels between Ang Ⅱ +Ezrin siRNA group and Ang Ⅱ +Ezrin siRNA+ALLN group.Conclusion Ezrin might play an important role in Ang Ⅱ-induced ABCA1 protein attenuation via calpain-1 pathway.关键词
埃兹蛋白/钙激活中性半胱氨酸蛋白酶/三磷酸腺苷结合盒转运体A1/血管紧张素ⅡKey words
Ezrin/Ca2+-dependent cysteine proteinase/Adenosine triphosphate binding cassette transporter A1/Angiotensin Ⅱ分类
医药卫生引用本文复制引用
王兰,郭静,莫显刚,安静,张莉,洪伟..Ezrin基因沉默通过降低Calpain-1活性抑制血管紧张素Ⅱ诱导的ABCA1降解[J].中国动脉硬化杂志,2018,26(3):217-221,5.基金项目
国家自然科学基金(31260250和31660288)资助项目 （31260250和31660288）
