中国动脉硬化杂志2018，Vol.26Issue(3)：222-226,5.
Wnt/β-catenin信号通路通过下调自噬改善大鼠脑缺血再灌注损伤
Wnt/β-catenin signaling pathway improves cerebral ischemia-reperfusion injury by down-regulation of autophagy in rats
摘要
Abstract
Aim To observe the effect of Wnt/β-catenin signaling pathway on cerebral ischemia-reperfusion (CIR) injury by regulating the autophagy level of rat cortical neurons.Methods 56 male SD rats were randomly divided into 4 groups:sham operation group (Sham group),CIR group,Wnt/β-catenin agonist lithium chloride (LiCl) treatment group (LiCl+CIR group),normal saline (NS) treatment group (NS+CIR group),14 rats in each group.Rat CIR damage model was established by middle cerebral artery occlusion with line embolus,with reperfusion 24 hours after ischemia 2 hours.Garcia neurological deficit score was used to evaluate the neurobehavioral changes of rats in each group.Cerebral infarction volume was measured by triphenyltetrazolium chloride staining.The expressions of β-catenin,LC3-Ⅱ and P62 proteins in rat cortex was detected by Western blot.Results After reperfusion for 24 h,compared with Sham group,the neurobehavioral score increased,the cerebral infarction volume increased,the cortical expression of β-catenin decreased,the cortical expression of LC3-Ⅱ increased,and the expression of P62 protein decreased in CIR group (P＜0.05).Compared with CIR group,the neurobehavioral score decreased,the cerebral infarction volume decreased,the cortical expression of β-catenin increased,the cortical expression of LC3-Ⅱ decreased,and the expression of P62 protein increased in LiCl+CIR group (P＜0.05).Conclusion Wnt/β-catenin signaling pathway can improve the nerve injury by regulating autophagy in CIR rats.关键词
Wnt/β-catenin信号通路/脑缺血再灌注/自噬/皮层神经元/脑保护Key words
Wnt/β-catenin signaling pathway/Cerebral ischemia-reperfusion/Autophagy/Cortical neuron/Cerebral protection分类
医药卫生引用本文复制引用
耿武军,陈成宇,张民远,莫云长,唐红丽,王均炉,俞卫锋..Wnt/β-catenin信号通路通过下调自噬改善大鼠脑缺血再灌注损伤[J].中国动脉硬化杂志,2018,26(3):222-226,5.基金项目
国家自然科学基金(81774109、81603685) （81774109、81603685）
