中国动脉硬化杂志2018，Vol.26Issue(3)：237-244,8.
加减枳实薤白桂枝汤通过AC/cAMP通路对缺血再灌注大鼠心肌组织水通道蛋白表达的影响
Effect of modified Zhishi Xiebai Guizhi decoction on the expression of aquaporin in myocardial tissue of ischemia-reperfusion rats through AC/cAMP pathway
摘要
Abstract
Aim To investigate the effect of modified Zhishi Xiebai Guizhi decoction (Zhishi,Xiebai,Sanqi,Renshen,etc.) on the expression of aquaporin (AQP) in myocardial tissue of ischemia-reperfusion rats and its possible mechanism.Methods 52 SD rats were randomly divided into 4 groups:sham operation group (n =10),model group (n =14),Zhishi Xiebai Guizhi decoction group (ZXGD group,n =14) and modified Zhishi Xiebai Guizhi decoction group (mZXGD group,n =14).Left anterior descending coronary artery was ligated after 14 days of intragastric administration,and the myocardial ischemia-reperfusion injury model was established according to the method of ischemia 30 minutes and reperfusion 120 minutes.Changes of serum lactate dehydrogenase (LDH) and cardiac troponin Ⅰ (cTnⅠ) were detected by automatic biochemical analyzer.Change of cardiac histopathology was observed by HE staining.The levels of plasma adenylate cyclase (AC) and cyclic adenosine monophosphate (cAMP) were detected by enzyme-linked immunosorbent assay.Immunohistochemical staining was used to detect the positioning expressions of AQP1 and AQP4 in myocardial tissue.AQP1 and AQP4 mRNA expressions in myocardial tissue was detected by real-time fluorescence quantitative PCR.Western blot was used to detect protein expressions of protein kinase A (PKA),phosphorylated PKA (P-PKA),cAMP-response element binding protein (CREB),phosphorylated CREB (P-CREB),AQP1 and AQP4 in myocardial tissue.Results Compared with sham operation group,the contents of serum LDH,cTnⅠ and plasma AC,cAMP were increased (P＜0.01),and the expressions of P-PKA,P-CREB,AQP1,AQP4 mRNAs and proteins in myocardial tissue were increased (P＜0.01),in model group.Compared with model group,the contents of serum LDH,cTnⅠ and plasma AC,cAMP were decreased (P＜0.01),and the expressions of P-PKA,AQP1,AQP4 mRNAs and proteins in myocardial tissue were decreased (P＜ 0.01),in ZXGD group and mZXGD group.Compared with ZXGD group,the contents of serum LDH,cTnⅠ and plasma AC,cAMP were decreased (P＜0.01),and the expressions of P-PKA,AQP1,AQP4 mRNAs and proteins in myocardial tissue were decreased (P＜ 0.01),in mZXGD group.Conclusion Modified Zhishi Xiebai Guizhi decoction can improve the pathological damage of myocardial cells after ischemia-reperfusion,and its mechanism may be related to down-regulating the AC/cAMP pathway and inhibiting AQP1,AQP4 mRNAs and proteins expression.关键词
加减枳实薤白桂枝汤/腺苷酸环化酶/环磷酸腺苷通路/心肌缺血再灌注损伤/水通道蛋白Key words
Modified Zhishi Xiebai Guizhi decoction/AC/cAMP pathway/Myocardial ischemia reperfusion injury/Aquaporin分类
医药卫生引用本文复制引用
李楠楠,石月萍..加减枳实薤白桂枝汤通过AC/cAMP通路对缺血再灌注大鼠心肌组织水通道蛋白表达的影响[J].中国动脉硬化杂志,2018,26(3):237-244,8.基金项目
辽宁省科学技术基金资助项目(201602307) （201602307）
