中国动脉硬化杂志2018，Vol.26Issue(3)：245-252,8.
p15与c-Myc在TGF-β1诱导的大鼠心肌细胞肥大中的表达及与TGF/Smad信号通路的关系
Study on p15 and c-Myc with TGF/Smad signal pathway of cardiomyocyte hypertrophy induced by TGF-β1 in rat
摘要
Abstract
Aim To investigate pathway mechanism about transforming growth factor-β1 (TGF-β1) activated cardiomyocyte hypertrophy.Methods Fresh isolated neonatal rat cardiomyocytes induced by TGF-β1 were used to examine formation of cell pathway.p-Smad2,Smad2,Smad2/3,p15 and c-Myc proteins were measured by Western blot.Cardiomyocyte hypertrophy was measured by PI statining and RNA expressions by real-time PCR.Smad signal pathway expression and it's related protein expression were induced by TGF-β1 and siRNA interference.Results TGF-β1 could induce the expression of hypertrophic gene in cultured cardiomyocytes in vitro,and the expression of β-MHC was significantly higher than that of the control group (P＜0.01).The content of RNA in TGF-β1 group was significantly increased by PI staining (P ＜0.01).Compared with the control group,TGF-β1 significantly increased the expression of p15 and c-Myc in p-Smad2,Smad2,Smad2/3 and Smad pathway (P＜0.01).The expression of p15 of Smad2 siRNA group was less than that of TGF-β1 group (P＜0.01).Conclusion TGF-β1 may induce cardiomyocyte hypertrophy through the Smad protein pathway,and in this process the expression of p15 is increased.关键词
转化生长因子β1/心肌细胞肥大/TGF/Smad信号通路/p15/c-MycKey words
Transforming growth factor-β1/Cardiomyocyte hypertrophy/TGF/Smad signal pathway/p15/c-Myc分类
医药卫生引用本文复制引用
石永英,卢俊江,陈广原,刘世明,陈敏生..p15与c-Myc在TGF-β1诱导的大鼠心肌细胞肥大中的表达及与TGF/Smad信号通路的关系[J].中国动脉硬化杂志,2018,26(3):245-252,8.基金项目
广东省医学科学技术研究基金项目(A2016376) （A2016376）
