中国骨伤2018，Vol.31Issue(4)：379-385,7.DOI:10.3969/j.issn.1003-0034.2018.04.016
锂剂抑制脊髓损伤后大鼠细胞凋亡的机制研究
Depressant effect of Lithium on apoptosis of nerve cells of adult rats after spinal cord injury
摘要
Abstract
Objective:To study whether lithium agent produces neuroprotective effect by inhibiting the nerve cell apoptosis of rats after spinal cord injury.Methods:Forty-two male SD rats weighing 200 to 250 g were randomly divided into 3 groups:blank control group (n=6) without surgery,normal saline (NS) group (n=18) with intraperitoneal injection of NS (40 mg/kg);and Lithium chloride (Licl) group (n=18) with intraperitoneal injection of Licl (40 mg/kg).After Alien method modeling,Licl group started intraperitoneal injection of Licl solution (40 mg· kg-1· d-1) within 15 min after operation to the second week.NS group,during the same interval,was injected with a same amount of NS.Postoperative 3,7,14 d,BBB scores in each group were measured;the expression of Bcl-2 and Bax protein were observed by immunohistochemisty staining;TUNEL staining was used to observe the nerve cell apoptosis.Results:The BBB scores in blank control group were 21.Postoperative 7,14 d,BBB scores of Licl group were higher than that of NS group (P＜0.05).As for the Bcl-2 protein expression,black control group has a level of 0.081 ±0.003;7 d and 14 d postoperatively,the level in Licl group was 0.151 ±0.003,0.163±0.003 and in NS group,0.143±0.003,0.154±0.002,respectively.Licl group showed significantly increased Bcl-2 protein expression (P＜0.05).As for the Bax protein expression,black control group showed a level of 0.071±±0.003;7 d and 14 d postoperatively,the level in Licl group was 0.121 ±±0.002,0.106±0.002 and in NS group was 0.126±0.001,0.120±0.002,respectively.The Bax protein expression is significantly inhibited in the Licl group (P＜0.05).In nerve cell apoptosis by TUNEL staining,the positive cells were fewer in the black control group with apoptosis index (AI) of 1.98±0.19;while 7d and 14d postoperatively,the AI of Licl group was 13.12±0.69,4.29±1.00 and of NS group,18.26±0.87,5.48±0.70,respectively.Licl group showed significant inhibition of the cell apoptosis (P＜0.05).Conclusion:Licl can promote the Bcl-2 protein expression and inhibit the Bax proteins expression in nerve cells of rat after SCI,thereby playing a role in the inhibition of nerve cell apoptosis.This may be one of the mechanisms that Licl can promote the recovery of motor function of rats after SCI.关键词
脊髓损伤/锂剂/神经元/细胞凋亡Key words
Spinal cord injury/Lithium/Neurons/Apoptosis引用本文复制引用
王放,周超,高正超,李宇欢,杨文龙,王栋,李浩鹏,贺西京..锂剂抑制脊髓损伤后大鼠细胞凋亡的机制研究[J].中国骨伤,2018,31(4):379-385,7.基金项目
国家自然科学基金(编号:81701223) （编号:81701223）
陕西省自然科学基金(编号:2017JQ8019) （编号:2017JQ8019）
中央高校基本科研业务费专项资金资助(编号:1191329737)Natural Science Foundation of China(No.81701223) （编号:1191329737）
