局解手术学杂志2018，Vol.27Issue(4)：235-240,6.DOI:10.11659/jjssx.01E018111
低频脉冲超声对小鼠海马区小胶质细胞的影响
Effect of low intensity pulsed ultrasound on the morphology of microglia in hippocampus of mice
摘要
Abstract
Objective To study the effect of low intensity pulsed ultrasound on the hippocampus and to study its possible mechanism in mice.Methods Totally 32 adult C57/BL6 mice were randomly divided into the sham group(n=16)and the LIPUS group(n=16).Mice in the sham group were treated with sham operative,while mice in the LIPUS group were treated by low intensity pulsed ultrasound(average power density of 150 mW/cm2).After 7 days of treatment,immunohistochemical(IHC)and western bolt(WB)were used to determine the expression of DCX,GFAP,and Iba1 in the hippocampus,and the behavioral changes of mice in open field test were observed.Results After 7 days of treatment,the results of IHC showed that the number of microglial cells in the LIPUS group were significantly increased,and the difference was statistically significant compared with that in the sham group(P<0.05).The WB results indicated that the expression of Iba 1 protein in hippocampus was significantly increased in LIPUS group compared with that in the sham group(P<0.05).In the meantime,the behavior score of LIPUS group in the open field test was increased significantly(P<0.05).Conclusion Low intensity pulsed ultrasound can increase the number of microglia in the hippocampus and reduce the anxiety of the mice.关键词
低频脉冲超声/小胶质细胞/海马/焦虑状态Key words
low intensity pulsed ultrasound/microglia/hippocampus/anxiety分类
医药卫生引用本文复制引用
周家兴,余晓娇,陈西,林森,叶剑..低频脉冲超声对小鼠海马区小胶质细胞的影响[J].局解手术学杂志,2018,27(4):235-240,6.基金项目
国家自然科学基金(81570804 （）
81371006) （）
重庆市院士牵头科技创新引导专项(cstc2017jcyj-yszxX0006) （cstc2017jcyj-yszxX0006）
大坪眼科预研基金(9-2543) （9-2543）
