中国临床药理学杂志2018，Vol.34Issue(3)：275-278,4.DOI:10.13699/j.cnki.1001-6821.2018.03.026
肝缺血再灌注损伤对大鼠心肾能量代谢的研究
Study of hepatic ischemia-reperfusion injury on cardiac and renal energy metabolism in rats
摘要
Abstract
Objective To study the influence of hepatic ischemia reperfusion injury (IRI)on cardiac and renal energy metabolism in rats.Methods The rats were randomly divided into five groups:the sham operation group,model-0 h group,model-2 h group,model-4 h group,model-6 h group,and ten rats in each group.The hepatic IRI rats model was prepared with non traumatic vascular clamp.The animal were sacrificed immediately after IRI 0,2,4,6 h and samples were collected.The tissue content of Na +-K +-ATPase and Ca2 +-Mg2 +-AT-Pase were detected by enzyme linked immunosorbent assay.Results The Ca2 +-Mg2 +-ATPenzyme of liver tissue in sham operation group,model-2 h group,model-4 h group were (343.84 ± 22.11),(221.88 ±64.15),(235.02 ± 58.84) μmol · g-1;compared with the sham operated group,the differences were statistically significant (all P＜0.05).The Na+-K +-ATP enzyme of renal tissuein sham operation group,model-2 h group,model-4 h group were (79.46 ± 10.84),(67.28 ± 3.39),(69.09 ± 1.47) μmol·g-1.The Ca2+-Mg2+-ATP enzyme of renal tissue in the three groups were (345.68 ± 21.68),(227.39 ± 50.63),(246.38 ± 64.31) μmol · g-1;compared with the sham operated group,the difference was statistically significant (all P ＜ 0.05).The Na +-K +-ATP enzyme of myocardium tissue in sham operation group,model -0 h group,model-2 h group,model-4 h group,model-6 h groupwere (68.16 ± 12.17),(71.92 ± 9.91),(61.62 ±7.96),(59.23 ±3.86),(79.72 ±9.91) μrnol · g-1.The Ca2+-Mg2+-ATP enzyme of myocardium tissue in the five groups were (261.05 ±40.52),(279.13 ±34.12),(243.62 ± 23.59),(244.66 ± 17.79),(291.78 ± 36.38) μmol · g-1;compared with the sham operated group,the differences were statistically significant (all P ＜ 0.05).There were no obvious changes in myocardial tissue energy metabolism indexes.Conclusion From energy metabolism indicators,when there is a hepatic ischemia reperfusion injury,the functions of the heart and kidney are all damaged,but the injury of kidney is earlier than that of myocardium.关键词
蒙医五行/肾、肝、心的母子关系/肝缺血再灌注/能量代谢Key words
five elements of Mongolian medicine/mother child relationship of kidney, liver, and heart/hepatic ischemia -reperfusion/energy metabolism分类
医药卫生引用本文复制引用
黎明,巴图德力根,马凤艳,那日苏,韩志强..肝缺血再灌注损伤对大鼠心肾能量代谢的研究[J].中国临床药理学杂志,2018,34(3):275-278,4.基金项目
内蒙古自治区医疗卫生科研计划基金资助项目(201302120) （201302120）
