摘要
Abstract
Objective To investigate the effect of tetramethylpyrazine in combination with cisplatin on the expression of vascular endothelial growth factor (VEGF) and Galectin-1 on Lewis lung cancer in mice.Methods A transplanted tumor model of Lewis lung cancer was established.Fourty mouse were randomly divided into four groups (n =10):blank group (0.9％ NaC1,0.2 mL),tetramethylpyrazine group (100 mg · kg-1,0.2 mL),cisplatin group(2 mg · kg-1,0.2 mL),and united group(the doses as above).Different treatments were served for 14 days,then all of rats were put to death to calculate inhibitory rate.The expression of VEGF and Galectin-1 were decteted by immunohistochemistry.Results The growth inhibition rate (GIR) in tetramethylpyrazine group,cisplatin group,and united group were 20.42％,34.65％,59.81％.The expression of VEGF and Galectin-1:blank group,tetramethylpyrazine group,cisplatin group,and united group were (62.41 ± 8.83) × 103,(27.84±2.25) × 103,(28.67 ±2.33) × 103,(23.52 ±2.76) ×103;(25.96 ±3.50) × 103,(20.29 ± 2.85) × 103,(19.11 ± 2.42) × 103,(15.15 ± 2.43) × 103 respectively.Compared with blank group,the differnces in the three drugs groups were significant (all P ＜ 0.01).Compared with tetramethylpyrazine group the differnces in united group were significant (all P ＜ 0.01).Compared with cisplatin group,the difference of Galectin-1 and VEGF in united group were significant (P ＜ 0.05,P ＜ 0.01).The expression of VEGF had positive correlation with Galectin-1 (r =0.638,P ＜ 0.05).Conclusion Tetramethylpyrazine can effectively inhibit the growth of lewis lung cancer transplanted tumor,which has synergetic effect when used in combination with cisplatin.The mechanism might be that it can inhibit tumor angiogenesis by reducing VEGF and Galectin-1 expression.关键词
川芎嗪/顺铂/肺癌/血管内皮生长因子/半乳糖凝集素1Key words
tetramethylpyrazine/cisplatin/lung cancer/vascular endothelial growth factor/Galectin-1分类
医药卫生
