摘要
Abstract
Objective To explore the inhibitory effect of bruceolic oil emulsion on lung carcinoma cell A549 and the related mechanism.Methods The lung carcinoma cell A549 managed by different final mass concentration bruceolic oil emulsion (0.50,1.25,2.50 g · L-1) were acted as low,medium,high concentration test groups,at the same time the control group was managed by equivalent 0.9％ NaCl.The morphological changes of lung carcinoma cell A549 in each group were detected by 4,6-diamino-2-phenyl indole (DAPI) staining.The proliferation inhibition and the expression of anti-apoptosis protein Survivin was detected by methyl thiazolyl tetrazolium (MTT) and Western blot respectively.Results At 24 h after intervention,the proliferation inhibition rates of control group and low,medium,high concentration test groups were (0.31 ± 0.19) ％,(37.24 ± 6.21) ％,(49.33 ± 5.96) ％,(61.25 ± 6.23) ％.The proliferation inhibition rates at 48 h after intervened were (0.32 ± 0.21) ％,(58.14 ± 6.33) ％,(65.28 ± 5.79) ％,(73.46 ± 5.37) ％.The proliferation inhibition rates at 72 h after intervened were (0.38 ±0.23)％,(68.58 ±5.87)％,(76.35 ±6.02)％,(83.97 ± 5.64)％.Compared with control group,the proliferation inhibition rate of lung carcinoma cell A549 in test groups increased significantly (P ＜ 0.01),and the proliferation inhibition rates showed a trend of gradually increasing with the increasing of bruceolic oil emulsion concentration and the extension of action time (P ＜ 0.05 or P ＜ 0.01).The Western blot detection results showed that,the Survivin protein grey value ratio of control group and low,medium,high concentration test groups were 0.95 ± 0.07,0.83 ± 0.10,0.66 ± 0.12,0.51 ± 0.09.Compared with control group,the expression of Survivin protein of lung carcinoma cell A549 in test groups decreased significantly (all P ＜ 0.01),and the expression of Survivin protein showed a trend of gradually decrease with the increasing of bruceolic oil emulsion concentration (P ＜ 0.01).Conclusion Bruceohc oil emulsion can inhibit the proliferation of lung carcinoma cell A549 effectively,and induce its apoptosis;the related action mechanism maybe be it can inhibit the expression of anti-apoptosis Survivin protein.关键词
肺癌细胞A549/鸦胆子油乳/增殖/凋亡/抑制Key words
lung carcinoma cell A549/bruceolic oil emulsion/proliferation/apoptosis/inhibition分类
医药卫生
