中国临床药理学杂志2018，Vol.34Issue(3)：297-299,311,4.DOI:10.13699/j.cnki.1001-6821.2018.03.033
虎杖苷对脓毒血症肾损伤大鼠的肾小球血管内皮的保护作用
Protective effect of polydatin on glomerular endothelial cells in septic rats with renal injury
摘要
Abstract
Objective To investigate the protective effects of polydatin on performance of sepsis injury of renal glomerular vascular endothelial and the activity of adenosine monophosphate (AMP)-activated protein kinase(AMPK) and mammalian target protein (rmTOR)in the process.Methods The sepsis model was established by injection of lipopolysaccharide through the tail vein of rats.The rats were divided into four groups:sham operated group (implementation of anesthesia,intubation),model group (anesthesia,intubation and sepsis),control group (anesthesia,intubation and application of saline resuscitation),experimental group (anesthesia,intubation and sepsis,saline combined with tiger polydatin 10 kU · kg-1).The activity of AMPK and rmTOR in each groups were detected by ELISA.Results After administration for 6 h,the heart rate (beat per minute) in experimental group,control group,model group were 88.21 ± 17,102.36 ± 12.83,118.04 ± 16.51.The urea nitrogen(BUN) in the three groups were (49.77 ±9.67),(77.92 ± 12.01),(122.19 ± 12.87) mg · dL-1.Compared with the control and model groups,all of them in experimental group were statistically significant (all P ＜ 0.05).The protein activeties of AMPK and mTOR in experimental group,model group were respectively 3.06 ± 1.34,0.77 ± 0.21;2.48 ± 1.12,3.95 ± 1.02.Compared with model groups,all of them in experimental group were statistically significant (all P ＜ 0.05).Conclusion Polydatin had protective effect on septic renal damage glomerular vascular endothelial,which might be correlated with its inhibition on mTOR activity through AMPK pathway.关键词
虎杖苷/脓毒血症/内皮细胞/磷酸腺苷活化蛋白激酶/西罗莫司靶点Key words
polydatin/sepsis/endothelial glycocalyx layer/AMP-activated protein kinase/mammalian target of rapamycin分类
医药卫生引用本文复制引用
刘世芬,丁玉峰,牛建一,丁刚,孙亮..虎杖苷对脓毒血症肾损伤大鼠的肾小球血管内皮的保护作用[J].中国临床药理学杂志,2018,34(3):297-299,311,4.基金项目
山东省自然科学基金青年基金资助项目(2014ZRB14193) （2014ZRB14193）
