中国临床药理学杂志2018，Vol.34Issue(3)：300-303,4.DOI:10.13699/j.cnki.1001-6821.2018.03.034
多靶点激酶抑制药GNF-7对白血病细胞表达谱的影响
Effect of multitargeted kinase inhibitor GNF-7 on the expression profiling of leukemia cell lines
摘要
Abstract
Objective To explore the effect of multitargeted kinase inhibitor GNF-7 on the expression profiling of leukemia cell lines.Methods The expression profiles dataset GSE49534 was downloaded from the Gene Expression Omnibus (GEO) database.The BRB-Array Tools software package was employed to screen the differentially expressed genes (DEGs),then the Gene Ontology (GO) function,pathway enrichment,gene interaction network,and pathway relation network analyses were conducted based on these differential genes.Results Totally,847 differential genes were screened out,of which 426 genes were up-regulated and 419 genes were down-regulated.GO enrichment analysis showed that DEGs mainly performed the molecular functions of binding,protein kinase activity,and signal transducer activity,and participated in biological process of signal transduction,small molecule metabolic process,and apoptotic process.The pathway analysis found that DEGs were mostly enriched in ribosome biogenesis in eukaryotes,metabolic pathways,Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway,etc.The network analyses mining identified the hub genes,which included polyribonucleotide nucleotidyltransferase 1 (PNPT1),adenylate kinase 4 (AK4),Janus kinase 2 (JAK2),signal transducer and activator of transcription 2 (STAT2),MYC;and core pathways such as mitogen-activated protein kinase (MAPK) signaling pathway,apoptosis,cell cycle,pathways in cancer.Conclusion The GNF-7 inhibited leukemia cells via induction of apoptosis and cell cycle arrest.关键词
白血病/靶向治疗/激酶抑制药/细胞凋亡/生物信息学Key words
leukemia/targeted treatment/kinase inhibitor/cell apoptosis/bioinformatics分类
医药卫生引用本文复制引用
荆凌华,刘松年,杨海平,阮林海..多靶点激酶抑制药GNF-7对白血病细胞表达谱的影响[J].中国临床药理学杂志,2018,34(3):300-303,4.基金项目
洛阳市科技计划基金资助项目(1503007A-4) （1503007A-4）
