中国临床药理学杂志2018，Vol.34Issue(3)：316-318,3.DOI:10.13699/j.cnki.1001-6821.2018.03.038
高效液相色谱法测定人血浆中伏立康唑的含量
Determination of the concentration of voriconazole in human plasma by HPLC
李敏 1陈超阳 2许俊羽 1马凌悦 1康子胜 1李欣然 3赵侠 1周颖 2崔一民1
作者信息
- 1. 北京大学第一医院药剂科,北京 100034
- 2. 北京大学药学院药事管理与临床药学系,北京 100191
- 3. 北京大学第一医院临床药理研究所,北京 100034
- 折叠
摘要
Abstract
Objective To establish a HPLC method for determining the concentration of voriconazole (VRC) in human plasma.Methods The analysis was conducted using a ZORBAX Eclipse Plus C18 column and the column temperature was 40 ℃.The mobile phase consisted of methanol and water (55∶45).The flow rate was 1 mL · min-1 and the detection was performed at 254 nm.And the internal standard substance was p-Chloroacetanilide.The specificity,lower limit of quantitation (LLOQ),standard curve,precision,accuracy,recovery and stability were investigated.Results Endogenous impurities did not interfere with the determination of the samples in plasma.The standard curve was linear in the range of 0.1-10.0 μg · mL-1 (r =0.999 2),LLOQ was 0.1 μg · mL-1.The RSDs of inter-day and intra-day were all less than 10％ in the plasma and the extraction recovery was 89％-92％.Conclusion This method was simple,sensitive,accurate,efficient and suitable for routine determination of the concentration of VRC in human plasma.关键词
伏立康唑/代谢物/高效液相色谱法/血浆Key words
voriconazole/metabolite/HPLC/plasma/saliva分类
医药卫生引用本文复制引用
李敏,陈超阳,许俊羽,马凌悦,康子胜,李欣然,赵侠,周颖,崔一民..高效液相色谱法测定人血浆中伏立康唑的含量[J].中国临床药理学杂志,2018,34(3):316-318,3.
