中国临床药理学杂志2018，Vol.34Issue(3)：319-322,4.DOI:10.13699/j.cnki.1001-6821.2018.03.039
超高效液相色谱串联质谱快速测定大鼠血浆的鸡骨草皂苷含量
Determination of the concentration of β-D-glucopyranosyl-3-(O-β-D-glucopyranosyl-oxy)-oleanolate in rat plasma by ultra performance liquid chromatography and tandem mass spectrometry
摘要
Abstract
Objective To determine the concentration ofβ-D-gluco-pyranosy1-3-(0-β-D-glucopyranosyloxy)-oleanolate (AA-2) in rat plasma by ultra performance liquid chromatography and tandem mass spectrometry (UPLC-MS/MS) method.Methods Plasmas were separated from the heparinized whole blood by centrifugation.After addition of chlorzoxazone as an internal standard (IS),protein precipitation by acetonitrile-methanol (9∶1) was used to prepare samples.Chromatographic separation was achieved on a UPLC BEH C18column (2.1 mm × 100 mm,1.7 μm) with 0.1％ formic acid and acetonitrile as the mobile phase with gradient elution.An electrospray ionization source was applied and operated in negative ion mode.Multiple reactions monitoring (MRM) mode was used for quantification using target fragment ions m/z 793.5→631.4 for AA-2,and m/z 168.1→132.1 for IS.Results Calibration plots were linear throughout the range of 10-5000 ng · mL-1 for AA-2 in rat plasma.Mean recoveries of AA-2 in rat plasma ranged from 70.4％ to 87.8％.RSD of intra-day and inter-day precision were both less than 10％.The accuracy of the method was between 91.1％ and 104.1％.After intravenous administration of AA-2(1,3,5 mg · kg-1),the mean residence time(MRT0-t) were (0.32 ±0.13),(0.48 ±0.15),(0.44±0.08) h.The i1/2 were (1.15±0.72),(1.32 ± 0.90),(1.02 ± 0.22) h.The CL were (4.53±1.66),(4.44 ±0.94),(4.86±0.49) L · h-1 · kg-1 The Cmax were (681.67 ±246.78),(1809.45 ±479.11),(4953.21 ± 722.72) ng· mL-1.Conclusion The method was simple,rapid and sensitive,which was suitable for pharmacokinetics study of AA-2 after intravenous administration in rats.关键词
鸡骨草皂苷/超高效液相串联三重四级杆质谱法/药代动力学/血药浓度Key words
Achyranthes aspera L saponin/UPLC-MS/MS/pharmacokinetics/plasma concentration分类
医药卫生引用本文复制引用
曹明,周云芳,谢文天..超高效液相色谱串联质谱快速测定大鼠血浆的鸡骨草皂苷含量[J].中国临床药理学杂志,2018,34(3):319-322,4.基金项目
浙江省医药卫生一般研究计划基金资助项目(2015KYB457) （2015KYB457）
