中国临床药理学杂志2018，Vol.34Issue(3)：323-326,4.DOI:10.13699/j.cnki.1001-6821.2018.03.040
反相高效液相色谱法测定人血浆百草枯浓度及其临床应用
Clinical application of determination of paraquat in human plasma by RP-HPLC
彭怡 1董维冲 1李浩然 1李德强 1张志清1
作者信息
- 1. 河北医科大学第二医院药学部,石家庄 050000
- 折叠
摘要
Abstract
Objective To establish a HPLC method for the determination of paraquat in human plasma which can be used in clinical practice.Methods The plasma samples were processed with WCX solid phase extraction column and were determinated by RP-HPLC.The HPLC determination was conducted on a Diamonsil C18 column (250 mm × 4.6 mm,5 μm)with the mobile phase consisted of 0.1 mol · L-1 phosphate buffer (containing 80 mmol · L-1 sodium heptanesulfonate,pH 3.0)-acetonitrile (80:20),and the flow rate was 1.0 mL · min-1.The detection wavelength was set at 258 nm,and the column temperature was 25 ℃.Results The plasma concentration of paraquat had good linearity over the range of 20-5000 ng· mL-1 (r =0.999 9).The limit of detection for the paraquat was 20 ng· mL-1.The relative recovery rates of paraquat were 90.06％-104.50％.The absolute recovery rates were 97.66％-99.11％,respectively.The intra-day and inter-day precision (RSD) were all less than 4.00％.The paraquat plasma sample was all stable at the conditions of maintaining at 4 ℃ for 8 h,after freeze thaw cycle and keeping at-40 ℃ for 21 days.The method was applied to the clinical determination of paraquat and the calculation of severityindex of paraquatpoison (SIPP),which was helpful in the clinical treatment of patients with paraquat poison and to predict the prognosis.Conclusion A rapid,sensitive and accurate RP-HPLC method was established,which can be used in the determination of paraquat in human plasma and predict the effect of clinical treatment of patients with paraquat poison.关键词
百草枯/血浆浓度/反相高效液相色谱法/临床应用Key words
paraquat/plasma concentration/RP-HPLC/clinical application分类
医药卫生引用本文复制引用
彭怡,董维冲,李浩然,李德强,张志清..反相高效液相色谱法测定人血浆百草枯浓度及其临床应用[J].中国临床药理学杂志,2018,34(3):323-326,4.
