中国临床药理学杂志2018，Vol.34Issue(5)：544-547,4.DOI:10.13699/j.cnki.1001-6821.2018.05.016
叶黄素对异丙肾上腺素诱导的大鼠心肌纤维化的抑制作用
Inhibitory effect of Lutein on myocardial fibrosis induced with isoprenaline in rats
摘要
Abstract
Objective To investigate the inhibitory effect and its mechanism of Lutein on myocardial fibrosis (MF) in rats.Methods Wistar rats were randomly divided into five groups according to the weight:normal group,model group,high,middle and low three doses of the experimental group (20,40,80 mg · kg-1 · d-1 Lutein),10 rats in each group.Except normal group,the rats in other groups were used to construct MF models by subcutaneous injection of isoproterenol (Iso:5 mg · kg-1 · d-1) for 7 days,and experimental groups were given different doses of Lutein from second days.Normal and model groups were given the same amount of distilled water.After the end of the experiment,heart mass index (HMI) and the left ventricular mass index (LVMI) were measured by analytical balance.Procollagen I C-terminal propeptide (PICP) and procollagen Ⅲ N-terminal propeptide (PmNP) were detected by ELISA method.The content of serum lactate dehydrogenase (LDH) and creatine kinase (CK) and nitric oxide(NO) level were detected by microplate reader.The activity of calcineurin (CaN)and the content of malondialdehyde (MDA) in myocardial tissue of rats were measured by ultraviolet spectra.Results Compared with normal group,in model group:HMI (mg · g-1) and LVMI (mg · g-1) were 3.73 ±0.58 and 2.57 ±0.43;PICP(ng · mL-1) and PⅢNP(ng · mL-1) were 7.27 ±0.33 and 24.86 ± 1.88,LDH and CK were 465.42 ±47.05 and 489.21 ±27.70;NO(μmol · L-1)was 2.06 ±0.34;MDA (nmol ·mg-1) content was 2.93 ±0.51 and the activity of CaN (U ·mg-1) was 0.14 ±0.02,the difference of above data between two groups were statistically significant (P ＜0.05 or P ＜0.01).Compared with the model group,experimental-L,-M,-H groups(unit:refer model group):HMI and LVMI were 3.13 ±0.40,3.02 ±0.28,2.82 ±0.22 and 2.19 ±0.27,2.09 ±0.27,1.99 ±0.12;the content of serum PICP and PⅢNP were 6.44 ±0.69,6.23 ±0.29,6.14 ±0.75 and 22.17 ±2.10,21.88 ± 1.93,21.76 ± 1.09;the content of LDH and CK in serum were 442.13 ± 33.72,390.19 ± 41.31,344.89 ± 50.25 and 442.89 ± 37.40,369.30 ± 28.46,346.57 ± 28.56;the level of NO in serum were 3.07 ± 0.45,4.59 ± 0.64,5.16 ± 0.52;the content of MDA and activity of CaN were 0.07 ± 0.01,0.06 ± 0.00,0.04 ± 0.01 and 2.48 ±0.27,2.44 ±0.17,2.25 ±0.11;these data were obviously significant (P ＜0.05 or P ＜0.01).Conclusion Lutein can be antioxidant and prevent calcium channel function by increasing the level of NO,reducing the content of MDA and the activity of the CaN to inhibit the synthesis of collagen and the myocardial fibrosis of rats to protect the myocardium.关键词
叶黄素/心肌纤维化/异丙肾上腺素/Ⅰ型前胶原羧基末端肽/乳酸脱氢酶Key words
Lutein/myocardial fibrosis/isoproterenol/procollagen I C-terminal propeptide/lactate dehydrogenase分类
医药卫生引用本文复制引用
赵丽晶,安英,陈雪,徐博,沈楠..叶黄素对异丙肾上腺素诱导的大鼠心肌纤维化的抑制作用[J].中国临床药理学杂志,2018,34(5):544-547,4.基金项目
吉林省教育厅“十二五”科学技术研究基金资助项目(2015396) （2015396）
吉林省“大学生创新创业训练计划”基金资助项目(2016B314) （2016B314）
