中国临床药理学杂志2018，Vol.34Issue(5)：552-554,3.DOI:10.13699/j.cnki.1001-6821.2018.05.018
不饱和脂肪酸对人宫颈癌细胞毒性作用机制研究
Toxicity mechanism of the unsaturated fatty acids on human cervical carcinoma of ME-180 cells
摘要
Abstract
Objective To investigate the cytotoxic effect mechanism of the unsaturated fatty acids on human cervical carcinoma of ME-180 cells.Methods The 2 × 108 cell · L-1 ME-180 cells were moved into the 96 hole culture plate,per hole 90 μL.Three concentrations of the drug groups:0.3,0.6,0.9 mg· L-1 unsaturated fatty acid (prepared with DMSO) per hole 90 μL.The 5-fluorouracil(5-FU) group:10mg · L-1 5-FU (prepared with phosphate buffer) per hole 10 μL.Control group:30 μL · L-1 DMSO per hole 10 μL.Each group set of four repeat hole,in saturated humidity condition of 37 ℃ and 5％ CO2 in 48 h.The inhibition rate and apoptosis rate of unsaturated fatty acids were detected with a trypan blue,thiazole blue (MTT),and flow cytometry method.Results When ME-180 cells were administered with the unsaturated fatty acids at the doses of 0.3,0.6,0.9 mg · L-1 for 48 h,ME -180 living cell count with the increase of drug concentration and action time is reduced.The cells proliferation inhibition rate were 43.35％,51.75％,66.45％ in three concentrations of the drug groups;apoptosis rate were 18.21％,23.11％,28.06％ in the three groups,respectively.Compared with the drug groups and the control group on the number of living cells,inhibition rate and apoptosis rate,the differences were statistically significant (P ＜0.01).Conclusion The unsaturated fatty acids has significant toxic effects on ME-180 cells.The toxic effect mechanism of the unsaturated fatty acids is related to inhibite proliferation on cell and induce-cell apoptosis.关键词
不饱和脂肪酸/宫颈癌细胞/毒性作用/抑制率/凋亡Key words
unsaturated fatty acids/ME-180 cells/toxic effect/inhibition rate/apoptosis分类
医药卫生引用本文复制引用
俞发荣,陈望军,连秀珍,谢明仁,李登楼,郭蕴萱,杨博..不饱和脂肪酸对人宫颈癌细胞毒性作用机制研究[J].中国临床药理学杂志,2018,34(5):552-554,3.基金项目
甘肃省基础研究创新群体基金资助项目(145RJIA333) （145RJIA333）
兰州市科技计划基金资助项目(2014-1-182 （）
2015-3-80) （）
甘肃省高校科技创新团队基金资助项目(2016C-09) （2016C-09）
甘肃省科技计划基金资助项目(17JR5RA158) （17JR5RA158）
兰州市人才创新创业基金资助项目(2016-RC-85) （2016-RC-85）
西北民族地区侦查理论与实务研究中心基金资助项目 （）
甘肃政法学院“文翰学者”基金资助项目. （）
