中国临床药理学杂志2018，Vol.34Issue(5)：562-564,575,4.DOI:10.13699/j.cnki.1001-6821.2018.05.021
四逆散对创伤后应激障碍所致睡眠障碍大鼠海马超微结构的影响
Effect of Sinisan on ultra structure of hippo-campus in the intervene rats by post traumatic stress disorder
摘要
Abstract
Objective To study the effect of Sinisan on the ultra structure of hippo-campus to the intervention in rats with post-traumatic stress disorder(PTSD).Methods SD rats were divided equally into 5 groups,each group had ten rats:blank control group,model group,negative control group,positive control group and experimental group.The blank control group did not copy the model,the normal feeding.Model group,repetitive post-traumatic stress disorder model was induced by current stimulation,but not treated.Negative control group,equal volume of 0.9％ NaC1.Positive control group (paroxetine hydrochloride 0.42 mg · mL-1) and experimentalgroup (Sinisan,containing crude drug 0.24 g · mL-1).It given to drugs 1 h before the model establishment.The rats were administered with 10 mL · kg-1,once a day,for a total of 7 d.In each group,rats was cardiac perfusion and the hippo-campus tissue was collected.The changes of ultra structure of CA1 and CA3 area in hippo-campus with rats were observed by transmission electron microscope.Results In the blank control group,the CA1 and CA3 neurons in the hippocampus were rich in cytoplasmic organelles,and the mitochondria were round or long,the structure of the mitochondrial cristae was clear,the rough endoplasmic reticulum was like a cord like distribution,and the ribosome was abundant.Compared with the blank control group,the organelle of CA1 and CA3 area in hippo-campus of the model group were significantly damaged,the cytoplasm was open,the mitochondria were swollen,the mitochondrial membrane and cristae disappeared,and the rough endoplasmic reticulum was dilated.The results showed that the damage of the organelles in the hippocampal neurons of rats was induced by the electric shock.The model group was similar to the negative control group,which indicated that there was no significant effect on the stress of rats after intragastric administration.The intracellular organelles in the CA1 and CA3 neurons in the hippocampus of the positive control group and the experimental group were significantly recovered,and the changes in the two groups were similar.These results suggest that both paroxetine hydrochloride and Sinisan can significantly improve the structure of CA1 and CA3 neurons in the hippocampus of PTSD rats.Conclusion Snisan as traditional Chinese medicine compound can significantly improve the hippocampus of rats with PTSD CA1 and CA3 neurons.关键词
四逆散/创伤后应激障碍/睡眠障碍/海马Key words
Sinisan/post-traumatic stress disorder/sleep disorder/hippocampus分类
医药卫生引用本文复制引用
李越峰,牛江涛,边甜甜,司昕蕾,徐富菊,严兴科..四逆散对创伤后应激障碍所致睡眠障碍大鼠海马超微结构的影响[J].中国临床药理学杂志,2018,34(5):562-564,575,4.基金项目
国家自然科学基金资助项目(81460611) （81460611）
国家教育部科学技术研究重点基金资助项目(212186) （212186）
甘肃省自然科学基金资助项目(2010:1010RJZA212 （）
2014:145RJZA076) （）
甘肃省财政厅高校基本科研业务费专项基金资金项目(2013-2) （2013-2）
兰州市科技计划基金资金项目(2014-1-188) （2014-1-188）
甘肃省中医药管理局科研课题基金资金项目(GZK-2015-57) （GZK-2015-57）
