中国临床药理学杂志2018，Vol.34Issue(5)：576-580,5.DOI:10.13699/j.cnki.1001-6821.2018.05.025
灯盏乙素对大鼠瑞舒伐他汀血浆浓度及组织分布的影响
Effect of scutellarin on the plasma concentration and tissue distributions of rosuvastatin in rats
摘要
Abstract
Objective To study the influence of scutellarin on the plasma concentration and tissue distributions of rosuvastatin in rats.Methods Male sprague-dawley rats were randomly divided into four groups:control group,experimental Ⅰ group,experimental Ⅱ group and experimental Ⅲ group.All rats were orally administrated with rosuvastatin (5 mg· kg-1).In low,medium,largethree doses (10,50,150 mg · kg-1 scutellarin) of experimental groups,scutellarin were added,respectively.Plasma samples were collected by picking eyeballs 1,2,6 h after administration.Then the rats were sacrificed and heart,liver,kidney,lung and brain were collected.The concentration of rosuvastatin in plasma and tissues were detected by HPLC-MS.Results The estabhshed HPLC-MS analyse method is in line with the requirements of this study.Concentrations of rosuvastatin in plasma at different time points were as follows:at 1 h it were (170.38 ± 40.77),(218.09 ± 59.52),(278.64 ± 76.26),(297.48 ± 95.37)ng.mL-1;at 2 h it were (237.26 ±70.31),(293.76 ±89.04),(410.27 ± 102.13),(461.49 ± 121.73)ng · mL-1;at 6 h it were (131.58 ±40.52),(166.21 ±48.52),(236.78 ±73.40),(251.33 ± 82.94) ng · mL-1.The concentration of rosuvastatin in experimental of rats plasma is higher than that of control group with significantly (all P ＜ 0.05).The rosuvastatin is mainly accumulated in the liver of the rats.Concentrations of rosuvastatin in liver at different time points were as follows:at 1 h it were (2098.57 ± 735.10),(1905.91 ± 703.16),(1406.39 ± 435.86),(1217.08 ± 318.65) ng · mL-1;at 2 h it were (3204.19 ± 893.53),(2863.87 ± 836.12),(2114.28 ± 598.31),(1973.15 ± 512.64) ng· mL-1;at 6 h it were (1681.71 ± 513.29),(1603.14 ± 517.86),(1120.08 ± 298.75),(1057.91 ± 267.66) ng · mL-1.In liver tissue,the concentration of rosuvastatin in experimental group H and Ⅲ were lower than that of the control group with significantly (all P ＜ 0.05).The concentration of rosuvastatin in heart,lung and kidney and brain tissue increased in experimental Ⅲ group;compared to that of the control group,the difference had significant difference (all P ＜ 0.05).Conclusion A specific concentration range of scutellarin can affect the plasma concentration and tissue distributions of rosuvastatin while rats were administrated with 5 mg · kg-1 of rosuvastatin.The concentration of rosuvastatin in liver under combined administration model was decreased,especially.This may be related to the competitive protein transporter.关键词
灯盏乙素/瑞舒伐他汀/组织分布/血药浓度Key words
scutellarin/rosuvastatin/tissue distributions/plasma concentration分类
医药卫生引用本文复制引用
刘建明,杨宇秀,叶颖俊,于海胜,刘志军,温金华..灯盏乙素对大鼠瑞舒伐他汀血浆浓度及组织分布的影响[J].中国临床药理学杂志,2018,34(5):576-580,5.基金项目
国家自然科学基金青年基金资助项目(81202583) （81202583）
江西省教育厅科技计划重点基金资助项目(GJJ151335) （GJJ151335）
