中国畜牧杂志2018，Vol.54Issue(4)：42-46,54,6.DOI:10.19556/j.0258-7033.2018-04-042
miRNA-200a通过靶向ZEB1参与调节鸡胸肌细胞增殖分化
Study on the Role of gga (Gallus Gallus)-miRNA-200a in Regulating Cell Differentiation and Proliferation of Chicken Breast Muscle by Targeting ZEB1
摘要
Abstract
A lot of studies have proved that Zinc finger E-box binding homeobox 1,ZEB1 played a major role in cell proliferation,cell survival and cell differentiation.However,the regulation of ZEB1 expression by microRNAs (miRNAs) in chicken breast muscle still remains unknown.This experiment examined the expression of ZEB1 in chicken breast tissues at 55 weeks and 20 weeks of age.In this study,TargetScan and miRDB software were used to predict the target miRNA of chicken ZEB1 gene,and ZEB1 wild-type and mutant double fluorescent reporter vector were constructed,the relationship between the potential miRNA and ZEB1 was verified using dual luciferase reporter assay in chicken DF1 cells.Dual-luciferasereporter assays results demonstrated that the expression of luciferase reporter gene linked with part sequence of the YUTR of chicken ZEB1 gene was down-regulated by the overexpression of gga-miR-200a in the DF1 cells,and the down-regulation behavior was abolished when the gga-miR-200a binding site in 3'UTR of ZEB1 was mutated,indicating that gga-miR-200a can suppress the expression level of its target gene ZEB1.Therefore,we concluded that gga-miR-200a might be involved in the cell differentiation and proliferation of chicken breast muscle through binding with ZEB1 3'UTR.These findings will contribute to further understanding of the regulatory mechanisms of miRNAs in chickens.关键词
鸡/ZEB1/miR-200a/细胞增殖/细胞分化Key words
Chicken/ZEB1/miR-200a/Cell proliferation/Cell differentiation分类
农业科技引用本文复制引用
蒋可人,马峥,孙君卫,李芳,刘小军,康相涛,孙桂荣..miRNA-200a通过靶向ZEB1参与调节鸡胸肌细胞增殖分化[J].中国畜牧杂志,2018,54(4):42-46,54,6.基金项目
国家自然科学基金(31572356) （31572356）
河南省产学研合作项目(162107000066) （162107000066）
河南省基础与前沿技术研究(162300410162) （162300410162）
