中国畜牧杂志2018，Vol.54Issue(4)：83-88,6.DOI:10.19556/j.0258-7033.2018-04-083
腺病毒与慢病毒感染猪原代细胞的比较研究
Comparative Study on Adenovirus and Lentivirus Infection of Porcine Primary Cells
摘要
Abstract
This study was conducted to explore the optimal multiplicity of infection (MOI) value and harvest time of adenovirus and lentivirus infection porcine primary cells,in order to determine efficient methods for virus infection.Porcine skeletal muscle satellite cells,porcine preadipocytes and porcine bone mesenchymal stem cells were separately isolated and incubated in vitro.Adenovirus (MOI =0,50,100,200,500,1 500)and lentivirus (MOI =0,30,50,80,100,300) were respectively added to infect porcine primary cells,and the expression of green fluorescent protein were observed every 24 h using a fluorescent microscope.Results showed:when adenoviral MOI =500 or lentiviral MOI =80,after 4 d infection,the fluorescent intensity in three types of porcine primary cells was considerably strong and the cell forms remained intact.Comparatively,adenovirus could infect porcine skeletal muscle satellite cells more rapidly and efficiently.As for porcine preadipocytes and porcine bone mesenchymal stem cells (BMSCs),the infection speed of two types of virus was close,while the infection efficiency and the fluorescent intensity of lentivirus were both higher than adenovirus.Overall,adenovirus is more suitable as the gene transduction vector for porcine skeletal muscle satellite cells,while lentivirus is more efficient to infect porcine preadipocytes and porcine BMSCs.关键词
猪骨骼肌卫星细胞/猪前体脂肪细胞/猪骨髓间充质干细胞/腺病毒/慢病毒/MOIKey words
Porcine skeletal muscle satellite cells/Porcine preadipocytes/Porcine bone mesenchymal stem cells/Adenovirus/Lentivirus/MOI分类
农业科技引用本文复制引用
姚永曦,吴睿帆,汪以真,王新霞..腺病毒与慢病毒感染猪原代细胞的比较研究[J].中国畜牧杂志,2018,54(4):83-88,6.基金项目
浙江省自然科学基金(LZ17C170001) （LZ17C170001）
