中华耳科学杂志2018，Vol.16Issue(2)：216-220,5.DOI:10.3969/j.issn.1672-2922.2018.02.017
c-Maf基因在小鼠耳蜗内外毛细胞中的表达及意义
Expression and Significance of c-Maf in Inner and Outer Hair Cells in Mice
摘要
Abstract
Objective To investigate the expression,distribution and significance of c-Maf protein in inner and out-er hair cells in the mouse cochlea.Methods(1)Inner and outer hair cells in adult mice were collected individually ac-cording to their morphological differences,and tested for RNA level c-Maf gene by GeneChip microarray.(2)Distribu-tion and expression of different developmental stages of the c-Maf protein in inner and outer hair cells on the basilar membrane were examined and recorded by confocal microscopy with immunofluorescence,while quantitative analysis of c-Maf protein was performed using the Image Pro Plus software.Results(1)The level of c-Maf gene RNA was simi-lar in cochlear inner and outer hair cells;(2)c-Maf protein was expressed in the cytoplasm of inner and outer hair cells with similar expression intensity,although expression of the protein in the infranuclear region was stronger than in the supper nuclear region in inner hair cells;(3)c-Maf protein was stably expressed in inner and outer hair cells on Day 0,7 and 12 after the birth. Conclusions The c-Maf protein is stably expressed in cochlear hair cells after birth and highly concentrated in the subnuclear region in inner hair cells in adult mice.It seems that the c-Maf protein may play an im-portant role in cellular development and function in inner and outer hair cells.关键词
c-Maf/耳蜗/毛细胞/表达/发育Key words
c-Maf/Cochlea/Inner and Outer Hair Cells/Expression/Development分类
医药卫生引用本文复制引用
宋青玲,曲腾飞,张伟,于树夔,何志洲,龚树生..c-Maf基因在小鼠耳蜗内外毛细胞中的表达及意义[J].中华耳科学杂志,2018,16(2):216-220,5.基金项目
国家自然科学基金(81770996) Project supported by the National Nature Science Foundation of China(Grant No.81770996). （81770996）
