中成药2018，Vol.40Issue(4)：771-776,6.DOI:10.3969/j.issn.1001-1528.2018.04.002
大黄总蒽醌提取物对脑缺血再灌注损伤的保护作用及其机制
Protective effect and mechanism of total anthraquinone extract of Rhei Radix et Rhizoma on cerebral ischemia-reperfusion injury
摘要
Abstract
AIM To investigate the effect and mechanism of total anthraquinone extract of Rhei Radix et Rhizoma on cerebral ischemia-reperfusion injury and to provide relevant data references for its promising use in the management of cerebral ischemia-reperfusion injury.METHODS SD rats randomly assigned to model group,sham operation group,nimodipine group,total anthraquinone extract groups (high,medium and low dose),8 in each group,were orally administered with corresponding drugs daily for a week,with rats of the model group and sham operation group given the same volume of normal saline before the models established by middle cerebral artery occlusion (MCAO).MCAO started thirty minutes after final oral administration,and the induced ischemia went on for 1.5 h for a further reperfusion,24 h after which the neurological function score,brain index,brain water content and cerebral infarct volume were measured.Elisa kits were used to detect superoxide dismutase (SOD),Malondialdehyde (MDA),Nitric oxide (NO),interleukin-1 β (IL-1β),tumor necrosis factor (TNF),interleukin-6 (IL-6).RESULTS The total anthraquinone extract of Rhei Radix et Rhizoma significantly improved the neurological function score,decreased the brain index,brain water content,reduced the cerebral infarct volume (P ＜ 0.05),increased the activity of SOD in brain tissue (P ＜ 0.01),and reduced the levels of MDA and NO in brain tissue (P ＜0.01),and the levels of IL-6,TNF and IL-1β in serum (P ＜0.01) as well.CONCLUSION The obviously protective effect on rats' cerebral ischemia-reperfusion injury by total anthraquinone extract of Rhei Radix et Rhizoma may contribute to its inhibition of inflammatory response,and its existence as an antioxidant as well.关键词
大黄/总蒽醌/炎症反应/抗氧化/脑缺血再灌注Key words
Rhei Radix et Rhizoma/total anthraquinone/inflammatory response/antioxidant/cerebral ischemia-reperfusion分类
医药卫生引用本文复制引用
万浩芳,万海同,周惠芬,虞立,陈烨,胡锦祥,石森林..大黄总蒽醌提取物对脑缺血再灌注损伤的保护作用及其机制[J].中成药,2018,40(4):771-776,6.基金项目
国家自然科学基金资助项目(81630105,81374053) （81630105,81374053）
